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Literature summary for 3.1.3.1 extracted from

  • von Tigerstrom R.G.; Stelmaschuk, S.
    Purification and characterization of the outer membrane associated alkaline phosphatase of Lysobacter enzymogenes (1986), J. Gen. Microbiol., 132, 1379-1387.
No PubMed abstract available
Search for more literature for 3.1.3.1

Inhibitors

Inhibitors Comment Organism Structure
8-hydroxyquinoline
-
 Lysobacter enzymogenes
EDTA
-
 Lysobacter enzymogenes
phosphate insensitive Lysobacter enzymogenes

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.034
-
 p-nitrophenyl phosphate and a second Km value of 0.056 mM Lysobacter enzymogenes
0.056
-
 p-nitrophenyl phosphate and a second Km value of 0.034 mM Lysobacter enzymogenes

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane enzyme is associated with the outer membrane Lysobacter enzymogenes 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
69000
-
 2 * 69000, SDS-PAGE of the heat-denatured enzyme Lysobacter enzymogenes
76000
-
 2 * 76000, SDS-PAGE of the enzyme without heat treatment Lysobacter enzymogenes
150000
-
 gel filtration Lysobacter enzymogenes

Organism

Organism UniProt Comment Textmining
Lysobacter enzymogenes
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Lysobacter enzymogenes

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Lysobacter enzymogenes

Storage Stability

Storage Stability Organism
-22°C, 10 mM Tris/HCl, 50 mM NaCl, 1.5 mM MgCl2, 0.2% Zwittergent 3-14, pH 8.4, stable for several weeks Lysobacter enzymogenes
0-4°C, 10 mM Tris/HCl, 50 mM NaCl, 1.5 mM MgCl2, 0.2% Zwittergent 3-14, pH 8.4, stable for several days Lysobacter enzymogenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2',3'-GMP + H2O
-
 Lysobacter enzymogenes ?
-
 ?
2',3'-UMP + H2O
-
 Lysobacter enzymogenes ?
-
 ?
2'-AMP + H2O
-
 Lysobacter enzymogenes adenosine + phosphate
-
 ?
2-phosphoglycerate + H2O
-
 Lysobacter enzymogenes glycerol + phosphate
-
 ?
3'-AMP + H2O
-
 Lysobacter enzymogenes adenosine + phosphate
-
 ?
3-phosphoglycerate + H2O
-
 Lysobacter enzymogenes glycerol + phosphate
-
 ?
5'-AMP + H2O
-
 Lysobacter enzymogenes adenosine + phosphate
-
 ?
5'-CMP + H2O
-
 Lysobacter enzymogenes cytidine + phosphate
-
 ?
5'-dAMP + H2O
-
 Lysobacter enzymogenes ?
-
 ?
5'-dCMP + H2O
-
 Lysobacter enzymogenes ?
-
 ?
5'-dGMP + H2O
-
 Lysobacter enzymogenes ?
-
 ?
5'-dTMP + H2O
-
 Lysobacter enzymogenes ?
-
 ?
5'-GMP + H2O
-
 Lysobacter enzymogenes guanosine + phosphate
-
 ?
5'-UMP + H2O
-
 Lysobacter enzymogenes uridine + phosphate
-
 ?
ADP + H2O
-
 Lysobacter enzymogenes ?
-
 ?
ATP + H2O
-
 Lysobacter enzymogenes ?
-
 ?
Glucose 6-phosphate + H2O
-
 Lysobacter enzymogenes Glucose + phosphate
-
 ?
p-nitrophenyl phosphate + H2O
-
 Lysobacter enzymogenes p-nitrophenol + phosphate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 69000, SDS-PAGE of the heat-denatured enzyme Lysobacter enzymogenes
dimer 2 * 76000, SDS-PAGE of the enzyme without heat treatment Lysobacter enzymogenes

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 stable for at least 20 min Lysobacter enzymogenes
70
-
 30% loss of activity after 10 min, 98% loss of activity after 20 min Lysobacter enzymogenes

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Lysobacter enzymogenes