Cloned (Comment) | Organism |
---|---|
expression in Pichia pastoris | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.94 | - |
p-nitrophenyl phosphate | - |
Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Homo sapiens | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
62000 | - |
x * 62000 or x * 65000, the purified recombinant enzyme appears in two subunit sizes, 62000 Da and 65000 Da, due to heterogenous N-glycosylation, SDS-PAGE | Homo sapiens |
65000 | - |
x * 62000 or x * 65000, the purified recombinant enzyme appears in two subunit sizes, 62000 Da and 65000 Da, due to heterogenous N-glycosylation, SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
side-chain modification | the purified recombinant enzyme appears in two subunit sizes, 62000 Da and 65000 Da, due to heterogenous N-glycosylation. There are two putative N-glycosylation sites in the primary sequence, Asn122-Thr-Thr and Asn249-Arg-Thr, only the latter appears to be glycosylated in the naive enzyme | Homo sapiens |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
placenta | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
p-nitrophenyl phosphate + H2O | - |
Homo sapiens | p-nitrophenol + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 62000 or x * 65000, the purified recombinant enzyme appears in two subunit sizes, 62000 Da and 65000 Da, due to heterogenous N-glycosylation, SDS-PAGE | Homo sapiens |