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Literature summary for 3.1.3.1 extracted from

  • Chaudhuri, G.; Chatterjee, S.; Venu-Babu, P.; Ramasamy, K.; Thilagaraj, W.R.
    Kinetic behaviour of calf intestinal alkaline phosphatase with pNPP (2013), Indian J. Biochem. Biophys., 50, 64-71.
    View publication on PubMed

Application

Application Comment Organism
analysis enzyme is capable of precipitating Cu2+ from 50 ppm CuCl2 solution with 64.3% precipitation. Phosphate generated from enzyme-mediated hydrolysis of 4-nitrophenyl phosphate binds with the copper ions Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.4
-
4-nitrophenyl phosphate 37°C, pH 9.5, glycine-NaOH buffer Bos taurus
0.76
-
4-nitrophenyl phosphate 37°C, pH 11, Tris-HCl buffer Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
calf
-

Source Tissue

Source Tissue Comment Organism Textmining
intestine
-
Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
Bos taurus 4-nitrophenol + phosphate
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
at pH 11 Bos taurus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
42.55
-
4-nitrophenyl phosphate 37°C, pH 9.5, glycine-NaOH buffer Bos taurus
82.98
-
4-nitrophenyl phosphate 37°C, pH 11, Tris-HCl buffer Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
glycine-NaOH buffer Bos taurus
11
-
Tris-HCl buffer Bos taurus