Literature summary for 3.1.3.1 extracted from

Schrenkhammer, P.; Rosnizeck, I.C.; Duerkop, A.; Wolfbeis, O.S.; Schaeferling, M.
Time-resolved fluorescence-based assay for the determination of alkaline phosphatase activity and application to the screening of its inhibitors (2008), J. Biomol. Screen., 13, 9-16.

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Application

Application	Comment	Organism
biotechnology	an assay is developed for determination of the activity of the ALP using the effect of enhancement of fluorescence of the europium(III)-tetracycline 3:1 complex (Eu(3)TC). Its luminescence, peaking at 616 nm if excited at 405 nm, is enhanced by a factor of 2.5 in the presence of phosphate. Phosphate coordinates to Eu(3)TC and enhances its luminescence intensity as a result of the displacement of water from the inner coordination sphere of the central metal. The assay is performed in a time-resolved (gated) mode, which is shown to yield larger signal changes than steady-state measurement of fluorescence. The limit of detection for ALP is 4 micromol/l. Based on this scheme, a model assay for theophylline as inhibitor for ALP is developed with a linear range from 14 to 68 micromol/l of theophylline	Bos taurus

Inhibitors

Inhibitors	Comment	Organism	Structure
theophylline	-	Bos taurus

Organism

Organism	UniProt	Comment	Textmining
Bos taurus	P19111	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
intestine	-	Bos taurus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-nitrophenyl phosphate + H2O	-	Bos taurus	4-nitrophenol + phosphate	-	?

Synonyms

Synonyms	Comment	Organism
alkaline phosphatase	-	Bos taurus
ALP	-	Bos taurus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	assay at	Bos taurus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	assay at	Bos taurus

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Release 2023.1 (January 2023)