Cloned (Comment) | Organism |
---|---|
expressed as a His6-tagged fusion protein in Escherichia coli | Thermus thermophilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 2 mM, 16% inhibition | Thermus thermophilus | |
Co2+ | 2 mM, 25% inhibition | Thermus thermophilus | |
Cu2+ | 2 mM, 29% inhibition | Thermus thermophilus | |
Fe2+ | 2 mM, 89% inhibition | Thermus thermophilus | |
Mn2+ | 2 mM, 32% inhibition | Thermus thermophilus | |
Zn2+ | 2 mM, 51% inhibition | Thermus thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.034 | - |
4-nitrophenyl phosphate | 75°C | Thermus thermophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 0.1 mM, 2.4fold increase in activity | Thermus thermophilus | |
Fe2+ | 0.1 mM, 1.25fold increase in activity | Thermus thermophilus | |
Mg2+ | 0.1 mM, 1.3fold increase in activity. 2 mM, 1.6fold increase in activity | Thermus thermophilus | |
Mn2+ | 0.1 mM, 1.3fold increase in activity | Thermus thermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54700 | - |
x * 54700, calculated from sequence | Thermus thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | Q153J0 | - |
- |
Thermus thermophilus XM | Q153J0 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl phosphate + H2O | - |
Thermus thermophilus | 4-nitrophenol + phosphate | - |
? | |
4-nitrophenyl phosphate + H2O | - |
Thermus thermophilus XM | 4-nitrophenol + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 54700, calculated from sequence | Thermus thermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | 80 | - |
Thermus thermophilus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 90 | 45°C: about 50% of maximal activity, 90°C: about 70% of maximal activity | Thermus thermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
270 min, about 10% loss of activity | Thermus thermophilus |
80 | - |
retains more than 50% activity after incubating for 6 h at 80ºC | Thermus thermophilus |
95 | - |
60 min, about 45% loss of activity | Thermus thermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
12 | - |
- |
Thermus thermophilus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
11 | 12.5 | 11.0: about 50% of maximal activity, pH 12.5: about 65% of maximal activity | Thermus thermophilus |