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Literature summary for 3.1.3.1 extracted from
- Characterization of a thermostable alkaline phosphatase from a novel species Thermus yunnanensis sp. nov. and investigation of its cobalt activation at high temperature (2005), Biochim. Biophys. Acta, 1750, 103-111.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.062 | - |
4-nitrophenyl phosphate | pH 9.5, 70°C, wild-type enzyme | Thermus yunnanensis |  |
| 1.43 | - |
4-nitrophenyl phosphate | pH 9.5, 70°C, Co2+-combined enzyme | Thermus yunnanensis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | activity is dependent on metal ion, Co2+ is the most active stimulator and has unique effect at high temperature | Thermus yunnanensis | |
| Mg2+ | activity is dependent on metal ion, Co2+, Mg2+ and Zn2+ are the main activators | Thermus yunnanensis | |
| Zn2+ | activity is dependent on metal ion, Co2+, Mg2+ and Zn2+ are the main activators | Thermus yunnanensis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 52000 | - |
2 * 52000, SDS-PAGE | Thermus yunnanensis |
| 104000 | - |
- |
Thermus yunnanensis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermus yunnanensis | - |
sp. nov. | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermus yunnanensis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3200 | - |
- |
Thermus yunnanensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl phosphate + H2O | - |
Thermus yunnanensis | 4-nitrophenol + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 52000, SDS-PAGE | Thermus yunnanensis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
- |
Thermus yunnanensis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 95 | 50°C: about 40% of maximal activity, 95°C: about 50% of maximal activity | Thermus yunnanensis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
half-life: 803 min | Thermus yunnanensis |
| 70 | - |
half-life: 527 min | Thermus yunnanensis |
| 80 | - |
half-life: 199 min, Mg2+ can increase half-life of the enzyme twice | Thermus yunnanensis |
| 90 | - |
half-life: 18 min | Thermus yunnanensis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 76 | - |
4-nitrophenyl phosphate | pH 9.5, 70°C, wild-type enzyme | Thermus yunnanensis | |
| 1130 | - |
4-nitrophenyl phosphate | pH 9.5, 70°C, Co2+-combined enzyme | Thermus yunnanensis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.5 | - |
- |
Thermus yunnanensis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | 10.5 | pH 8.5: about 50% of maximal activity, about 55% of maximal activity | Thermus yunnanensis |
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