Crystallization (Comment) | Organism |
---|---|
10-16 mg/ml purified enzyme in 10 mM Tris, pH 7.0, 2 mM MgCl2, 0.02% NaN3, sitting drop vapour diffusion method, free or in complex with substrate 4-nitrophenyl phosphate, reservoir solution: 12-14% PEG 4000, 20% PEG 3350, 2 mM zinc acetate, 100 mM sodium cacodylate, pH 6.5, the buffer can also be acetate, citrate or imidazole malate, X-ray diffraction structure determination and analysis at 1.9 A resolution | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-amino acids | specific uncompetitive inhibition, molecular mechanism involves Arg166 and Glu429 | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | metalloenzyme, metal binding site structure | Homo sapiens | |
Zn2+ | metalloenzyme, metal binding site structure | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P05187 | humanplacenta alkaline phosphatase PLAP | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | gylcosylation site Asn249 | Homo sapiens |
Purification (Comment) | Organism |
---|---|
from placenta | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a phosphate monoester + H2O = an alcohol + phosphate | Wide specificity. Also catalyses transphosphorylations. Some enzymes hydrolyse diphosphate, active site structure, highly conserved in evolution of alkaline phosphatases | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
placenta | tissue-specific enzyme | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl phosphate + H2O | allosteric mechanism | Homo sapiens | 4-nitrophenol + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | crystal structure | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
alkaline phosphatase | - |
Homo sapiens |
AP | - |
Homo sapiens |
PLAP | - |
Homo sapiens |