Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL-21cells | Xenotropic MuLV-related virus |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 27% (w/v) polyethylene glycol 1500 and 0.1 M sodium citrate, pH 4.7 | Xenotropic MuLV-related virus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-[(4'-aminomethyl-1,1'-biphenyl)methyl]-1-hydroxy-1,8-naphthyridin-2-one | - |
Xenotropic MuLV-related virus | |
7-(furan-2-yl)-2-hydroxy-isoquinoline-1,3(2H,4H)-dione | YLC2-155 | Xenotropic MuLV-related virus | |
trihydroxy benzoyl biphenyl carboxylate hydrazone | BHMP07 | Xenotropic MuLV-related virus | |
trihydroxybenzoyl naphthyl hydrazone | - |
Xenotropic MuLV-related virus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | optimal RNase H activity in the presence of Mn2+ and not Mg2+ | Xenotropic MuLV-related virus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
RNA-DNA hybrid + H2O | Xenotropic MuLV-related virus | - |
ribonucleotide 5'-phosphomonoester + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Xenotropic MuLV-related virus | A1Z651 | clone VP62 | - |
Purification (Comment) | Organism |
---|---|
GST column chromatography | Xenotropic MuLV-related virus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
RNA-DNA hybrid + H2O | - |
Xenotropic MuLV-related virus | ribonucleotide 5'-phosphomonoester + ? | - |
? | |
RNA-DNA hybrid HTS-1 + H2O | 5'-GAUCUGAGCCUGGGAGCU-fluorescein-3' annealed to 5'-Dabcyl-AGCTCCCAGGCTCAGATC-3' | Xenotropic MuLV-related virus | ? | - |
? | |
RNA-DNA hybrid HTS-2 + H2O | 5'-CUGGUUAGACCAGAUCUGAGCCUGGGAGCU-fluorescein-3' annealed to 5'-Dabcyl-AGCTCCCAGGCTCAGATC-3' | Xenotropic MuLV-related virus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
reverse transcriptase | possesses also RNase H activity | Xenotropic MuLV-related virus |
RNase H | - |
Xenotropic MuLV-related virus |
RNH | - |
Xenotropic MuLV-related virus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.000357 | - |
with RNA-DNA hybrid HTS-1 as substrate, at pH 7.8 and 37°C | Xenotropic MuLV-related virus | 4-[(4'-aminomethyl-1,1'-biphenyl)methyl]-1-hydroxy-1,8-naphthyridin-2-one | |
0.000402 | - |
with RNA-DNA hybrid HTS-2 as substrate, at pH 7.8 and 37°C | Xenotropic MuLV-related virus | 4-[(4'-aminomethyl-1,1'-biphenyl)methyl]-1-hydroxy-1,8-naphthyridin-2-one | |
0.01 | - |
IC50 above 0.01 mM, with RNA-DNA hybrid HTS-1 as substrate, at pH 7.8 and 37°C | Xenotropic MuLV-related virus | 7-(furan-2-yl)-2-hydroxy-isoquinoline-1,3(2H,4H)-dione | |
0.01 | - |
IC50 above 0.01 mM, with RNA-DNA hybrid HTS-1 as substrate, at pH 7.8 and 37°C | Xenotropic MuLV-related virus | trihydroxy benzoyl biphenyl carboxylate hydrazone | |
0.01 | - |
IC50 above 0.01 mM, with RNA-DNA hybrid HTS-1 as substrate, at pH 7.8 and 37°C | Xenotropic MuLV-related virus | trihydroxybenzoyl naphthyl hydrazone | |
0.01 | - |
IC50 above 0.01 mM, with RNA-DNA hybrid HTS-2 as substrate, at pH 7.8 and 37°C | Xenotropic MuLV-related virus | 7-(furan-2-yl)-2-hydroxy-isoquinoline-1,3(2H,4H)-dione | |
0.01 | - |
IC50 above 0.01 mM, with RNA-DNA hybrid HTS-2 as substrate, at pH 7.8 and 37°C | Xenotropic MuLV-related virus | trihydroxy benzoyl biphenyl carboxylate hydrazone | |
0.01 | - |
IC50 above 0.01 mM, with RNA-DNA hybrid HTS-2 as substrate, at pH 7.8 and 37°C | Xenotropic MuLV-related virus | trihydroxybenzoyl naphthyl hydrazone |