Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Aeropyrum pernix |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000361 | - |
5'-(6-carboxy-fluorescein)-cggagaugacgg-3'/5'-CCGTCTCTCCG-3' | pH 9.0, 30°C | Aeropyrum pernix |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | the enzyme exhibits the highest activity in the presence of 5 mM Mn2+, 1 mM Co2+, or 10 mM Mg2+, respectively. The specific activity of the enzyme determined with 5 mM MnCl2 is slightly higher than that determined with 10 mM MgCl2, and about 2 folds higher than that determined with 1 mM CoCl2 | Aeropyrum pernix | |
Mg2+ | the enzyme exhibits the highest activity in the presence of 5 mM Mn2+, 1 mM Co2+, or 10 mM Mg2+, respectively. The specific activity of the enzyme determined with 5 mM MnCl2 is slightly higher than that determined with 10 mM MgCl2, and about 2 folds higher than that determined with 1 mM CoCl2 | Aeropyrum pernix | |
Mn2+ | the enzyme exhibits the highest activity in the presence of 5 mM Mn2+, 1 mM Co2+, or 10 mM Mg2+, respectively. The specific activity of the enzyme determined with 5 mM MnCl2 is slightly higher than that determined with 10 mM MgCl2, and about 2 folds higher than that determined with 1 mM CoCl2 | Aeropyrum pernix |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aeropyrum pernix | Q9YET5 | - |
- |
Aeropyrum pernix DSM 11879 | Q9YET5 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Aeropyrum pernix |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5'-(6-carboxy-fluorescein)-cggagaugacgg-3'/5'-CCGTCTCTCCG-3' + H2O | the enzyme cleaves 12-bp RNA/DNA at multiple sites between the 3rd and 11th residues, but most preferentially at c10g11 and less preferentially at g5a6 and u7g8. The cleavage pattern of Mg2+-dependent activity is the same as that of Co2+-dependent activity, but different from that of Mn2+-dependent activity | Aeropyrum pernix | ? | - |
? | |
5'-(6-carboxy-fluorescein)-cggagaugacgg-3'/5'-CCGTCTCTCCG-3' + H2O | the enzyme cleaves 12-bp RNA/DNA at multiple sites between the 3rd and 11th residues, but most preferentially at c10g11 and less preferentially at g5a6 and u7g8. The cleavage pattern of Mg2+-dependent activity is the same as that of Co2+-dependent activity, but different from that of Mn2+-dependent activity | Aeropyrum pernix DSM 11879 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Ape-RNase HII | - |
Aeropyrum pernix |
RNase HII | - |
Aeropyrum pernix |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Aeropyrum pernix |
70 | - |
- |
Aeropyrum pernix |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 90 | 50°C: about 60% of maximal activity, 90°C: about 40% of maximal activity | Aeropyrum pernix |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
stabilization of the thermostable enzyme is enhanced with increased salt concentration (Mg2+ or K+) | Aeropyrum pernix |
70 | - |
fully stable at 70°C for at least 10 min under assay condition containing 100 mM MgCl2. Under the assay condition containing 10 mM MgCl2, little activity can be detected | Aeropyrum pernix |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
assay at | Aeropyrum pernix |
11 | - |
- |
Aeropyrum pernix |