Crystallization (Comment) | Organism |
---|---|
crystal structure is determined at 1.6 A resolution, crystals are grown by the sitting-drop vapor-diffusion method at 4°C | Sulfurisphaera tokodaii |
Protein Variants | Comment | Organism |
---|---|---|
R118A | the specific activities of R118A-RNase HI for both 12-bp RNA/DNA and RNA/RNA substrates are reduced by approximately 10fold as compared to those of the wild-type protein | Sulfurisphaera tokodaii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sulfurisphaera tokodaii | F9VN79 | - |
- |
Sulfurisphaera tokodaii 7 | F9VN79 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
RNA/DNA hybrid + H2O | - |
Sulfurisphaera tokodaii | ? | - |
? | |
RNA/DNA hybrid + H2O | - |
Sulfurisphaera tokodaii 7 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ribonuclease HI | - |
Sulfurisphaera tokodaii |
Sto-RNase HI | - |
Sulfurisphaera tokodaii |
type 1 ribonuclease H | - |
Sulfurisphaera tokodaii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Sulfurisphaera tokodaii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
78 | - |
Tm-value for mutant enzyme DELTAC6 | Sulfurisphaera tokodaii |
93 | - |
Tm-value for mutant enzyme C58/145A | Sulfurisphaera tokodaii |
102 | - |
Tm-value for wild-type enzyme. The thermostable protein is destabilized by elimination of the disulfide bond and C-terminal truncation | Sulfurisphaera tokodaii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
assay at | Sulfurisphaera tokodaii |