Cloned (Comment) | Organism |
---|---|
expression of recombinant chimeric and point mutant enzymes in Escherichia coli strain BL21(DE3), I53 and L56 point mutants are expressed insolubly in inclusion bodies | Chlorobaculum tepidum |
expression of recombinant chimeric mutant enzyme and of His-tagged mutant L56S in Escherichia coli strain BL21(DE3) | Thermus thermophilus |
Protein Variants | Comment | Organism |
---|---|---|
I53A | site-directed mutagenesis | Chlorobaculum tepidum |
I53D | site-directed mutagenesis | Chlorobaculum tepidum |
L56A | site-directed mutagenesis | Chlorobaculum tepidum |
L56D | site-directed mutagenesis | Chlorobaculum tepidum |
L56S | site-directed mutagenesis | Thermus thermophilus |
L56S | site-directed mutagenesis | Chlorobaculum tepidum |
additional information | construction of chimeric proteins of the Thermus thermophilus enzyme with the folding core of Chlorobium tepidum RNaseH, thermal stability analysis, overview | Thermus thermophilus |
additional information | construction of chimeric proteins of the Thermus thermophilus enzyme with the folding core of Chlorobium tepidum RNaseH, thermal stability analysis, overview | Chlorobaculum tepidum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | thermodynamic analyses of chimeric proteins, overview | Chlorobaculum tepidum | |
additional information | - |
additional information | thermodynamic analyses of mutant enzymes, overview | Thermus thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chlorobaculum tepidum | - |
- |
- |
Thermus thermophilus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant recombinant chimeric and point mutant enzymes from Escherichia coli strain BL21(DE3) by heparin affinity chromatography | Chlorobaculum tepidum |
recombinant recombinant chimeric mutant enzyme from Escherichia coli strain BL21(DE3) by heparin affinity chromatography, and His-tagged mutant L56S by nickel affinitychromatography | Thermus thermophilus |
Renatured (Comment) | Organism |
---|---|
recombinant I53 and L56 point mutants after being expressed insolubly in inclusion bodies in Escherichia coli | Chlorobaculum tepidum |
Synonyms | Comment | Organism |
---|---|---|
CtepRNH | - |
Chlorobaculum tepidum |
RNase H | - |
Thermus thermophilus |
RNase H | - |
Chlorobaculum tepidum |
TthRNH | - |
Thermus thermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
analysis of thermal unfolding, the folding core of Chlorobium tepidum RNaseH plays an important role in the unfolded state of this protein | Chlorobaculum tepidum |
additional information | - |
analysis of thermal unfolding, the folding core of Thermus thermophilus RNaseH plays an important role in the unfolded state of this protein | Thermus thermophilus |