Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli and in HeLa cell | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
A177T | mutation in subunit H2B associated with Aicardi-Goutieres' syndrome, near-normal activity | Homo sapiens |
G185V | mutation in subunit H2B associated with Aicardi-Goutieres' syndrome, near-normal activity | Homo sapiens |
G37S | mutation in subunit H2A associated with Aicardi-Goutieres' syndrome, greatly reduced activity and processivity | Homo sapiens |
K143I | mutation in subunit H2C associated with Aicardi-Goutieres' syndrome, near-normal activity | Homo sapiens |
K162T | mutation in subunit H2B associated with Aicardi-Goutieres' syndrome, near-normal activity | Homo sapiens |
R69W | mutation in subunit H2C associated with Aicardi-Goutieres' syndrome, 30-40% of wild-type activity | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | highest activity in presence of 5-10 mM | Homo sapiens | |
Mn2+ | 0.1-1 mM, 20-30% of maximum activity | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
isoform RNase H2 | - |
Homo sapiens | Q5TBB1 | subunit H2B; isoform RNase H2 | - |
Homo sapiens | Q8TDP1 | subunit H2C; isoform RNase H2 | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA12-RNA1-DNA27/DNA40 hybrid + H2O | - |
Homo sapiens | ? | enzyme cleaves RNA20/DNA20 hybrid and DNA12-RNA1-DNA27/DNA40 hybrid substrates with similar efficiency | ? | |
poly-rA/poly-dT + H2O | - |
Homo sapiens | ? | products are short oligonucleotides with very few intermediate-sized oligonucleotides | ? | |
RNA20/DNA20 hybrid + H2O | - |
Homo sapiens | ? | enzyme cleaves RNA20/DNA20 hybrid and DNA12-RNA1-DNA27/DNA40 hybrid substrates with similar efficiency | ? |
Subunits | Comment | Organism |
---|---|---|
trimer | heterotrimer of subunits H2A, H2B, H2C, SDS-PAGE and gel filtration | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
RNase H2 | - |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.2 | 8.8 | - |
Homo sapiens |