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Literature summary for 3.1.26.4 extracted from

  • Hu, D.; Pu, F.; Huang, Z.; Ren, J.; Qu, X.
    A quadruplex-based, label-free, and real-time fluorescence assay for RNase H activity and inhibition (2010), Chemistry, 16, 2605-2610.
    View publication on PubMed

Application

Application Comment Organism
analysis quadruplex-based fluorescence assay for sensitive, facile, real-time, and label-free detection of RNase H activity and inhibition by using a G-quadruplex formation strategy. A RNA-DNA substrate is prepared, with the DNA strand designed as a quadruplex-forming oligomer. Upon cleavage of the RNA strand by RNase H, the released G-rich DNA strand folds into a quadruplex in the presence of monovalent ions and interacts with a specific G-quadruplex binder, N-methyl mesoporphyrin IX, giving a dramatic increase in fluorescence and serving as a reporter of the reaction. The assay is simple in design, fast in operation and convenient Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
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Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
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Escherichia coli
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