Cloned (Comment) | Organism |
---|---|
overexpression in strain BL21(DE3) | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
RNase HI-dsRNA enzyme-inhibitor complexes with 9-mer and 10-mer RNA, sitting-drop vapor-diffusion method, 0.001-0.002 ml of 0.11-0.15 mM protein solution is mixed with an equal volume of reservoir solution containing 50 mM bis-Tris, pH 6.1, 12.5% PEG 3350, 25 mM NaCl, 2 mM MgCl2 and 1 mM TCEP, addition of 20 mM HEPES pH 8.0, 16% PEG 3350, molecular replacement, X-ray diffraction structure determination and anaylsis at 3.5-4.0 A resolution | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA-RNA hybrid + H2O | RNase H binds RNA-DNA hybrid and double-stranded RNA duplexes with similar affinity, but only cleaves the RNA in the former in a specific manner, substrate recognition, overview | Escherichia coli | DNA + 5'-phosphonucleotides | - |
? |
Synonyms | Comment | Organism |
---|---|---|
RNase HI | - |
Escherichia coli |