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Literature summary for 3.1.26.4 extracted from

  • Loukachevitch, L.V.; Egli, M.
    Crystallization and preliminary X-ray analysis of Escherichia coli RNase HI-dsRNA complexes (2007), Acta Crystallogr. Sect. F, 63, 84-88.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in strain BL21(DE3) Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
RNase HI-dsRNA enzyme-inhibitor complexes with 9-mer and 10-mer RNA, sitting-drop vapor-diffusion method, 0.001-0.002 ml of 0.11-0.15 mM protein solution is mixed with an equal volume of reservoir solution containing 50 mM bis-Tris, pH 6.1, 12.5% PEG 3350, 25 mM NaCl, 2 mM MgCl2 and 1 mM TCEP, addition of 20 mM HEPES pH 8.0, 16% PEG 3350, molecular replacement, X-ray diffraction structure determination and anaylsis at 3.5-4.0 A resolution Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA-RNA hybrid + H2O RNase H binds RNA-DNA hybrid and double-stranded RNA duplexes with similar affinity, but only cleaves the RNA in the former in a specific manner, substrate recognition, overview Escherichia coli DNA + 5'-phosphonucleotides
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Synonyms

Synonyms Comment Organism
RNase HI
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Escherichia coli