Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | - |
Homo sapiens | |
DTT | - |
Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in Escherichia coli BL21(DE3) | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C147A | site-directed mutagenesis, mutant is active under reducing and oxidizing conditions | Homo sapiens |
C147A/C148A | site-directed mutagenesis, mutant is active under reducing and oxidizing conditions | Homo sapiens |
C148A | site-directed mutagenesis, mutant is active under reducing and oxidizing conditions | Homo sapiens |
C191A | site-directed mutagenesis, mutant is active under reducing conditions, but shows poor activity under oxidizing conditions | Homo sapiens |
additional information | construction of deletion mutant H1[DELTA1-73] | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
N-ethylmaleimide | inhibits wild-type enzyme and deletion mutant H1[DELTA1-73] | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Homo sapiens | |
Mn2+ | - |
Homo sapiens | |
additional information | enzyme requires divalent cations | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | the enzyme is regulated by a unique redox switch formed by adjacent Cys147 and Cys148 | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
type 1 enzyme | - |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant enzymes from Escherichia coli BL21(DE3) | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
reaction velocity at oxidizing and reducing conditions | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme is regulated by a unique redox switch formed by adjacent Cys147 and Cys148 | Homo sapiens | ? | - |
? | |
additional information | formation of a disulfide bond, under oxidizing conditions, between Cys147 and Cys148 results in an inactive enzyme conformation | Homo sapiens | ? | - |
? | |
RNA*DNA hybrid + H2O | enzyme is active only under reducing conditions, wild-type enzyme and deletion mutant H1[DELTA1-73] are inactive under oxidizing conditions | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
RNase H1 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |