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Literature summary for 3.1.26.4 extracted from

  • ten Asbroek, A.L.M.A.; Van Groenigen, M.; Nooij, M.; Baas, F.
    The involvement of human ribonucleases H1 and H2 in the variation of response of cells to antisense phosphorothioate oligonucleotides (2002), Eur. J. Biochem., 269, 583-592.
    View publication on PubMed
Search for more literature for 3.1.26.4

Cloned(Commentary)

Cloned (Comment) Organism
expression of enzyme type 1 and 2 as GFP-fusion proteins in all 6 cell lines, transfection of cell lines with antisense oligodeoxynucleotides directed against RNA polymerase II, replication protein A, and Ha-ras, determination of response in expression levels of the enzyme type 1 and 2, overview Homo sapiens

General Stability

General Stability Organism
partially purified enzyme extract is labile against freezing and dilution, addition of 45% glycerol Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
antisense oligodeoxynucleotides directed against RNA polymerase II, replication protein A, and Ha-ras, determination of response in expression levels of the enzyme type 1 and 2, overview Homo sapiens
additional information presence of intrinsic cell-type specific factors affecting the activity and localization of type 2 enzyme Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
additional information enzyme type 1 of all cell lines and enzyme type 2 of 15PC3 are distributed throughout the whole cell, presence of intrinsic cell-type specific factors affecting the activity and localization of type 2 enzyme Homo sapiens
-
-
nucleus type 2 enzyme, restricted to, except in cell line 15PC3 Homo sapiens 5634
-

Metals/Ions

Metals/Ions Comment Organism Structure
KCl
-
 Homo sapiens
Mg2+
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens presence of intrinsic cell-type specific factors affecting the activity and localization of type 2 enzyme ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 type 1 and type 2 enzymes
-

Purification (Commentary)

Purification (Comment) Organism
partially Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
15PC3 cell prostate cancer cell line, high level in enzyme type 1 and 2 activity Homo sapiens
-
HEK-293 cell embryonal kidney cell line, high level in enzyme type 1 activity, low level in enzyme type 2 activity Homo sapiens
-
HeLa cell cervical carcinoma cell line, high level in enzyme type 1 activity, moderate level in enzyme type 2 activity Homo sapiens
-
HTB-82 cell rhabdomyosarcoma cell line, low level in enzyme type 1 and 2 activity Homo sapiens
-
MiaPaCa-2 cell pancreatic carcinoma cell line, low level in enzyme type 1 activity, high level in enzyme type 2 activity Homo sapiens
-
T-24 cell bladder carcinoma cell line, low level in enzyme type 1 activity, moderate level in enzyme type 2 activity Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 determination of RNA levels Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information presence of intrinsic cell-type specific factors affecting the activity and localization of type 2 enzyme Homo sapiens ?
-
 ?
RNA*DNA hybrid + H2O specific for the RNA moiety Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
ribonuclease H
-
 Homo sapiens
RNase H
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Homo sapiens