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Literature summary for 3.1.26.4 extracted from
- Expression, purification, and characterization of a recombinant ribonuclease H from Thermus thermophilus HB8 (1992), J. Biol. Chem., 267, 10184-10192.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Thermus thermophilus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| acetonitrile | 20%, 50% loss of activity | Thermus thermophilus |  |
| SDS | 0.002%, 50% loss of activity | Thermus thermophilus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Thermus thermophilus | |
| additional information | - |
additional information | - |
Escherichia coli | |
| 0.0005 | - |
M13 DNA/RNA | at 37°C | Thermus thermophilus | |
| 0.0011 | - |
M13 DNA/RNA | at 70°C | Thermus thermophilus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Escherichia coli | |
| Mg2+ | required | Thermus thermophilus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 21000 | - |
gel filtration | Thermus thermophilus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| Thermus thermophilus | - |
- |
- |
| Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermus thermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| M13 DNA/RNA + H2O | - |
Thermus thermophilus | ? | - |
? | |
| M13 DNA/RNA + H2O | - |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | ? | - |
? | |
| RNA-DNA hybrid + H2O | - |
Escherichia coli | ribonucleotide 5'-phosphomonoester | - |
? | |
| RNA-DNA hybrid + H2O | M13 DNA/RNA hybrid | Thermus thermophilus | ribonucleotide 5'-phosphomonoester | - |
? | |
| RNA-DNA hybrid + H2O | M13 DNA/RNA hybrid | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | ribonucleotide 5'-phosphomonoester | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 21000, SDS-PAGE | Thermus thermophilus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
- |
Thermus thermophilus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 48.2 | - |
melting temperature in presence of 1.2 M guanidinium hydrochloride | Escherichia coli |
| 55 | - |
10 min, 60% loss of activity | Escherichia coli |
| 82.1 | - |
melting temperature in presence of 1.2 M guanidinium hydrochloride | Thermus thermophilus |
| 90 | - |
10 min, stable | Thermus thermophilus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Thermus thermophilus | |
| additional information | - |
additional information | - |
Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | 9.5 | cleavage of M13 DNA/RNA hybrid | Thermus thermophilus |
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