Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | enzyme form H2 is inactive in absence of mercaptoethanol. No effect on enzyme form H1 | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Dextran | - |
Mus musculus | |
EDTA | - |
Mus musculus | |
Ethidium bromide | - |
Mus musculus | |
KCl | activity of enzyme form H1 decreases rapidly above 50 mM and becomes nearly abolished at 150 mM | Mus musculus | |
NaCl | activity of enzyme form H1 decreases rapidly above 50 mM and becomes nearly abolished at 150 mM | Mus musculus | |
NEM | enzyme form H2. No effect on enzyme form H1 | Mus musculus | |
NH4Cl | activity of enzyme form H1 decreases rapidly above 50 mM and becomes nearly abolished at 150 mM | Mus musculus | |
Nucleic acids | enzyme form H1 is more susceptible to inhibition than enzyme form H2 | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Mus musculus | |
0.0005 | - |
poly(rAdT) | lower than 0.0005 mM | Mus musculus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | enzyme form H2 is mostly inactive at low salt and requires 100-200 mM concentration for maximal activity. KCl is more efficient than NaCl | Mus musculus | |
Mg2+ | enzyme form H2: requirement for divalent metal ion can be satisfied only by Mg2+. Enzyme form H1: requirement for a divalent metal ion can be satisfied by Mg2+ or with a stronger preference with Mn2+ | Mus musculus | |
Mn2+ | enzyme form H1: requirement for a divalent metal ion can be satisfied by Mg2+ or with a stronger preference with Mn2+ | Mus musculus | |
NaCl | enzyme form H2 is mostly inactive at low salt and requires 100-200 mM concentration for maximal activity. KCl or NH4Cl is more efficient than NaCl | Mus musculus | |
NH4Cl | enzyme form H2 is mostly inactive at low salt and requires 100-200 mM concentration for maximal activity. NH4Cl is more efficient than NaCl | Mus musculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
x * 33000, enzyme form H2, SDS-PAGE | Mus musculus |
36000 | 40000 | enzyme form H2, gel filtration | Mus musculus |
105000 | 110000 | enzyme form H1, gel filtration | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial, two types of enzyme: H1 and H2 | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
Krebs ascites cell | - |
Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
poly(rAdT) + H2O | - |
Mus musculus | ? | - |
? | |
RNA-DNA hybrid + H2O | poly(rAdT) | Mus musculus | ribonucleotide 5'-phosphomonoester | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 33000, enzyme form H2, SDS-PAGE | Mus musculus |