Activating Compound | Comment | Organism | Structure |
---|---|---|---|
sulfhydryl reagent | required for maximal activity of the Mn2+-dependent enzyme and the Mg2+-dependent enzyme | Gallus gallus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | inhibition of Mn2+-dependent enzyme, no inhibition of the Mg2+-dependent enzyme | Gallus gallus | |
diphosphate | inhibition of Mn2+-dependent enzyme, no inhibition of the Mg2+-dependent enzyme | Gallus gallus | |
NaF | inhibition of Mg2+-dependent enzyme | Gallus gallus | |
NEM | 2 mM, 80% inhibition of Mn2+-dependent enzyme, complete inhibition of Mg2+-dependent enzyme | Gallus gallus | |
PCMB | 2 mM, complete inhibition of Mn2+-dependent enzyme and Mg2+-dependent enzyme | Gallus gallus | |
spermidine | inhibition of Mn2+-dependent enzyme, no inhibition of the Mg2+-dependent enzyme | Gallus gallus | |
spermine | inhibition of Mn2+-dependent enzyme, no inhibition of the Mg2+-dependent enzyme | Gallus gallus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | Mg2+-dependent enzyme requires 15-20 mM Mg2+ for maximal activity | Gallus gallus | |
Mg2+ | optimal activity with 10-15 mM | Gallus gallus | |
Mn2+ | 0.4 mM Mn2+ required for optimal activity, some activity with Mg2+ | Gallus gallus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
Mg2+-dependent enzyme, gel filtration | Gallus gallus |
120000 | - |
Mn2+-dependent enzyme, gel filtration | Gallus gallus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gallus gallus | - |
one Mn2+-dependent RNase H and one Mg2+-dependent RNase H | - |
Purification (Comment) | Organism |
---|---|
one Mn2+-dependent RNase H and one Mg2+-dependent RNase H | Gallus gallus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
embryo | - |
Gallus gallus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Gallus gallus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
RNA-DNA hybrid + H2O | - |
Gallus gallus | ribonucleotide 5'-phosphomonoester | a mixture of oligoribonucleotides with 5'-phosphate and 3'-hydroxyl terminus | ? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
Mn2+-dependent enzyme | Gallus gallus |
9.5 | - |
Mg2+-dependent enzyme | Gallus gallus |