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Literature summary for 3.1.26.4 extracted from
- Ribonuclease H from rat liver. I. Partial purification and characterization of nuclear ribonuclease H1 (1978), J. Biochem., 84, 385-393.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| (NH4)2SO4 | optimal concentration: 25 mM | Rattus norvegicus |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| DNA | single-stranded or double-stranded, very strong | Rattus norvegicus | |
| Polyribonucleotides | slight | Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| additional information | multiple form of ribonuclease H exists in different regions of the rat liver cell | Rattus norvegicus | - |
- |
| nucleus | nucleoplasm and nucleoli | Rattus norvegicus | 5634 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required, optimal concentration: 17 mM | Rattus norvegicus | |
| Mn2+ | optimal concentration 0.6 mM | Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 36000 | - |
gel filtration | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 50% v/v glycerol, stable for several months | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| RNA-DNA hybrid + H2O | poly(rUdA) | Rattus norvegicus | ribonucleotide 5'-phosphomonoester | oligoribonucleotides with 3'-hydroxyl and 5'-phosphate termini | ? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | 9 | - |
Rattus norvegicus |
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Release 2023.1 (January 2023)
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