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Literature summary for 3.1.26.4 extracted from

  • Karwan, R.; Wintersberger, U.
    In addition to RNase H(70) two other proteins of Saccharomyces cerevisiae exhibit ribonuclease H activity (1988), J. Biol. Chem., 263, 14970-14977.
    View publication on PubMed
Search for more literature for 3.1.26.4

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ divalent cation required Saccharomyces cerevisiae
Mn2+ Mn2+ is preferred over Mg2+ Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40300
-
 ribonuclease H(42), gel filtration Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharomyces cerevisiae

Reaction

Reaction Comment Organism Reaction ID
Endonucleolytic cleavage to a 5'-phosphomonoester random endonucleolytic cleavage Saccharomyces cerevisiae
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.9244
-
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
RNA-DNA hybrid + H2O poly(rCdG) Saccharomyces cerevisiae ribonucleotide 5'-phosphomonoester mixture of oligonucleotides, ranging in size from dinucleotides to larger than hexanucleotides. No mononucleotides can be detected ?
RNA-DNA hybrid + H2O poly(rAdT) Saccharomyces cerevisiae ribonucleotide 5'-phosphomonoester mixture of oligonucleotides, ranging in size from dinucleotides to larger than hexanucleotides. No mononucleotides can be detected ?

Synonyms

Synonyms Comment Organism
ribonuclease H(42)
-
 Saccharomyces cerevisiae