Activating Compound | Comment | Organism | Structure |
---|---|---|---|
sulfhydryl reagent | is required | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NEM | - |
Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required, optimal activity at 2-4 mM | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
strain B and strain D110 | - |
Purification (Comment) | Organism |
---|---|
strain B and strain D110 | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme cannot cleave the phosphodiester bond covalently linking ribonucleotides to DNA | Escherichia coli | ? | - |
? | |
RNA-DNA hybrid + H2O | digestion of more than 95% of the RNA in RNA-DNA hybrids to acid-soluble products | Escherichia coli | ribonucleotide 5'-phosphomonoester | oligoribonucleotides with 3'-hydroxyl and 5'-phosphate termini | ? | |
RNA-DNA hybrid + H2O | poly(rAdT) | Escherichia coli | ribonucleotide 5'-phosphomonoester | oligoribonucleotides with 3'-hydroxyl and 5'-phosphate termini | ? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9.1 | - |
Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.9 | 9.1 | pH 6.9: 50% of maximal activity, pH 7.5-9.1: optimum | Escherichia coli |