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Literature summary for 3.1.26.4 extracted from

  • Berkower, I.; Leis, J.; Hurwitz, J.
    Isolation and characterization of an endonuclease from Escherichia coli specific for ribonucleic acid in ribonucleic acid-deoxyribonucleic acid hybrid structures (1973), J. Biol. Chem., 248, 5914-5921.
    View publication on PubMed
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Activating Compound

Activating Compound Comment Organism Structure
sulfhydryl reagent is required Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
NEM
-
 Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required, optimal activity at 2-4 mM Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 strain B and strain D110
-

Purification (Commentary)

Purification (Comment) Organism
strain B and strain D110 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme cannot cleave the phosphodiester bond covalently linking ribonucleotides to DNA Escherichia coli ?
-
 ?
RNA-DNA hybrid + H2O digestion of more than 95% of the RNA in RNA-DNA hybrids to acid-soluble products Escherichia coli ribonucleotide 5'-phosphomonoester oligoribonucleotides with 3'-hydroxyl and 5'-phosphate termini ?
RNA-DNA hybrid + H2O poly(rAdT) Escherichia coli ribonucleotide 5'-phosphomonoester oligoribonucleotides with 3'-hydroxyl and 5'-phosphate termini ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 9.1
-
 Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
6.9 9.1 pH 6.9: 50% of maximal activity, pH 7.5-9.1: optimum Escherichia coli