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Literature summary for 3.1.26.4 extracted from

  • Wyers, F.; Huet, J.; Sentenac, A.; Fromageot, P.
    Role of DNA-RNA hybrids in eukaryotes. Characterization of yeast ribonuclease H1 and H2 (1976), Eur. J. Biochem., 69, 385-395.
No PubMed abstract available
Search for more literature for 3.1.26.4

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol optimal concentration is 1 mM Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ absolute requirement for divalent cations, preferably Mg2+, optimal activity at 25 mM Saccharomyces cerevisiae
Mn2+ cation requirement can be fullfilled to some extent by 2 mM Mn2+ Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20700
-
 ribonuclease H2, gel filtration Saccharomyces cerevisiae
21000
-
 x * 21000, ribonuclease H2, SDS-PAGE Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
 ribonuclease H2
-

Purification (Commentary)

Purification (Comment) Organism
ribonuclease H2 Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
RNA-DNA hybrid + H2O
-
 Saccharomyces cerevisiae ribonucleotide 5'-phosphomonoester a mixture of oligonucleotides with 5'-phosphate termini and only a minor proportion of 5'-mononucleotide ?

Subunits

Subunits Comment Organism
? x * 21000, ribonuclease H2, SDS-PAGE Saccharomyces cerevisiae