Protein Variants | Comment | Organism |
---|---|---|
additional information | the catalytically inactive mutant RNase3-Ala can bind the substrates like 22 nt ds-siRNA or 60 bp dsRNA, formation of high-molecular-mass RNA-protein complexes | sweet potato chlorotic stunt virus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | Mg2+ best supports the catalytic activity of enzyme RNase3 | sweet potato chlorotic stunt virus | |
Mn2+ | less efficient in activation of the enzyme compared to Mg2+, but binding of 21 nt small dsRNA molecules is most efficient in the presence of Mn2+ | sweet potato chlorotic stunt virus | |
additional information | no activation of the enzyme by Ca2+ | sweet potato chlorotic stunt virus | |
Zn2+ | less efficient in activation of the enzyme compared to Mg2+ | sweet potato chlorotic stunt virus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | sweet potato chlorotic stunt virus | the class 1 enzyme binds and processes small dsRNA molecules, it can cleave long dsRNA molecules, synthetic small interfering RNAs (siRNAs), and plant- and virus-derived siRNAs extracted from sweet potato plants | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
sweet potato chlorotic stunt virus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the class 1 enzyme binds and processes small dsRNA molecules, it can cleave long dsRNA molecules, synthetic small interfering RNAs (siRNAs), and plant- and virus-derived siRNAs extracted from sweet potato plants | sweet potato chlorotic stunt virus | ? | - |
? | |
additional information | the enzyme cleaves ds-siRNAs and microRNAs (miRNAs) with a regular A-form conformation, while asymmetrical bulges, extensive mismatches and 2'-O-methylation of ds-siRNA and miRNA interfer with processing, substrate specifiicty of the enzyme in processing small RNA duplexes, overview | sweet potato chlorotic stunt virus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the enzyme binds siRNA as a dimer | sweet potato chlorotic stunt virus |
Synonyms | Comment | Organism |
---|---|---|
class 1 RNase III | - |
sweet potato chlorotic stunt virus |
dsRNA-specific class 1 RNase III-like endoribonuclease | - |
sweet potato chlorotic stunt virus |
RNase3 | - |
sweet potato chlorotic stunt virus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8.5 | processing of long dsRNA by RNase3 is efficient at pH 7.5-8.5, ds-siRNA is processed more efficiently at pH 8.5 | sweet potato chlorotic stunt virus |
General Information | Comment | Organism |
---|---|---|
evolution | the virus-encoded RNase3 binds dsRNA as a dimer, which is the active form able to accommodate dsRNA binding and cleavage, and supports the classification of RNase3 as a class 1 RNase III endoribonuclease | sweet potato chlorotic stunt virus |
additional information | the enzyme binds siRNA as a dimer, which is the active form able to accommodate dsRNA binding and cleavage | sweet potato chlorotic stunt virus |
physiological function | the dsRNA-specific class 1 RNase III-like endoribonuclease encoded by sweet potato chlorotic stunt virus suppresses posttranscriptional gene silencing and eliminates antiviral defence in sweet potato plants in an endoribonuclease activity-dependent manner | sweet potato chlorotic stunt virus |