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Literature summary for 3.1.26.3 extracted from

  • Weinheimer, I.; Boonrod, K.; Moser, M.; Wassenegger, M.; Krczal, G.; Butcher, S.J.; Valkonen, J.P.
    Binding and processing of small dsRNA molecules by the class 1 RNase III protein encoded by sweet potato chlorotic stunt virus (2014), J. Gen. Virol., 95, 486-495.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
additional information the catalytically inactive mutant RNase3-Ala can bind the substrates like 22 nt ds-siRNA or 60 bp dsRNA, formation of high-molecular-mass RNA-protein complexes sweet potato chlorotic stunt virus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ Mg2+ best supports the catalytic activity of enzyme RNase3 sweet potato chlorotic stunt virus
Mn2+ less efficient in activation of the enzyme compared to Mg2+, but binding of 21 nt small dsRNA molecules is most efficient in the presence of Mn2+ sweet potato chlorotic stunt virus
additional information no activation of the enzyme by Ca2+ sweet potato chlorotic stunt virus
Zn2+ less efficient in activation of the enzyme compared to Mg2+ sweet potato chlorotic stunt virus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information sweet potato chlorotic stunt virus the class 1 enzyme binds and processes small dsRNA molecules, it can cleave long dsRNA molecules, synthetic small interfering RNAs (siRNAs), and plant- and virus-derived siRNAs extracted from sweet potato plants ?
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Organism

Organism UniProt Comment Textmining
sweet potato chlorotic stunt virus
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-
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the class 1 enzyme binds and processes small dsRNA molecules, it can cleave long dsRNA molecules, synthetic small interfering RNAs (siRNAs), and plant- and virus-derived siRNAs extracted from sweet potato plants sweet potato chlorotic stunt virus ?
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?
additional information the enzyme cleaves ds-siRNAs and microRNAs (miRNAs) with a regular A-form conformation, while asymmetrical bulges, extensive mismatches and 2'-O-methylation of ds-siRNA and miRNA interfer with processing, substrate specifiicty of the enzyme in processing small RNA duplexes, overview sweet potato chlorotic stunt virus ?
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?

Subunits

Subunits Comment Organism
More the enzyme binds siRNA as a dimer sweet potato chlorotic stunt virus

Synonyms

Synonyms Comment Organism
class 1 RNase III
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sweet potato chlorotic stunt virus
dsRNA-specific class 1 RNase III-like endoribonuclease
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sweet potato chlorotic stunt virus
RNase3
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sweet potato chlorotic stunt virus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8.5 processing of long dsRNA by RNase3 is efficient at pH 7.5-8.5, ds-siRNA is processed more efficiently at pH 8.5 sweet potato chlorotic stunt virus

General Information

General Information Comment Organism
evolution the virus-encoded RNase3 binds dsRNA as a dimer, which is the active form able to accommodate dsRNA binding and cleavage, and supports the classification of RNase3 as a class 1 RNase III endoribonuclease sweet potato chlorotic stunt virus
additional information the enzyme binds siRNA as a dimer, which is the active form able to accommodate dsRNA binding and cleavage sweet potato chlorotic stunt virus
physiological function the dsRNA-specific class 1 RNase III-like endoribonuclease encoded by sweet potato chlorotic stunt virus suppresses posttranscriptional gene silencing and eliminates antiviral defence in sweet potato plants in an endoribonuclease activity-dependent manner sweet potato chlorotic stunt virus