Cloned (Comment) | Organism |
---|---|
recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli BL21(DE3) Codon Plus | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of mutant enzymes containing inserts encoding the enzyme's heparin binding region HBR1, HBR2, or HBR3 by site-directed mutagenesis, cytotoxicity of wild-type and mutant enzymes to Beas-2B cells, overview | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | the enzyme interacts with membrane lipids | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant C-terminally His6-tagged enzyme from Escherichia coli BL21(DE3) Codon Plus by metal affinity chromatography | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
BEAS-2B cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme interacts with membrane lipids | Homo sapiens | ? | - |
? | |
additional information | enzyme assay with commercial yeast tRNA | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
RNase3 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
additional information | three putative multifunctional heparin binding regions, 34RWRCK38 (HBR1), 75RSRFR79 (HBR2), and 101RPGRR105 (HBR3), of hRNase3 are identified by silico sequence analysis and validated by in vitro activity assays, the heparin binding peptide containing HBR1 is a key element associated with heparan sulfate binding, cellular binding, and lipid binding activities. Comparisons of CHO cell binding and cytotoxicity to Beas-2B cells of wild-type and mutant enzymes, overview | Homo sapiens |
physiological function | the human enzyme has multiple functions including ribonucleolytic, heparan sulfate binding, cellular binding, endocytic, lipid destabilization, cytotoxic, and antimicrobial activities | Homo sapiens |