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Literature summary for 3.1.26.3 extracted from

  • Ando, Y.; Maida, Y.; Morinaga, A.; Burroughs, A.M.; Kimura, R.; Chiba, J.; Suzuki, H.; Masutomi, K.; Hayashizaki, Y.
    Two-step cleavage of hairpin RNA with 5' overhangs by human DICER (2011), BMC Mol. Biol., 12, 6.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
cloning of full-length cDNA of DICER1 protein from HeLa cell total RNA, and expression of N-terminally FLAG-tagged DICER1 protein in 293T cells Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens two-step cleavage of hairpin RNA with 5' overhangs ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9UPY3
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information two-step cleavage of hairpin RNA with 5' overhangs Homo sapiens ?
-
?
additional information recombinant DICER protein processes a hairpin RNA with 5' overhangs in vitro and generates an intermediate duplex with a 29 nt-5' strand and a 23 nt-3' strand. Longer 5' overhangs with stable stem structures can reduce the efficiency or rate of substrate cleavage. In vitro two-step processing of the 5'-end labelled pre-mmu-mir-1982 RNA by recombinant DICER protein, overview Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
Dicer
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
assay at Homo sapiens

General Information

General Information Comment Organism
physiological function DICER is an RNase III family endoribonuclease that processes precursor microRNAs and long double-stranded RNAs, generating microRNA duplexes and short interfering RNA duplexes with 20-23 nucleotides in length Homo sapiens