Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | less effective than Mg2+ | Thermotoga maritima | |
Mg2+ | activates optimally at 1 mM | Thermotoga maritima | |
Mn2+ | less effective than Mg2+ | Thermotoga maritima | |
additional information | the enzyme functions optimally at pH 8 and about 50-80 mM salt concentrations | Thermotoga maritima | |
Ni2+ | less effective than Mg2+ | Thermotoga maritima |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga maritima | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | substrate specificity with RNA duplex substrates, overview. The proximal box is a primary reactivity epitope for Tm-23S[hp] RNA. a CG or GC bp substitution at pb position 2 reduces the relative reactivity to 0.1 and 0.3, respectively, while a CG or GC substitution at pb position 4 provides a relative reactivity of 0.1 or 0.4, respectively. At pb position 3, only the AU bp substitution causes a significant drop in relative reactivity, while none of the bp substitutions at pb position 1 has a significant effect | Thermotoga maritima | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ribonuclease III | - |
Thermotoga maritima |
RNase III | - |
Thermotoga maritima |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 70 | RNase III catalytic activity exhibits a broad optimal temperature range | Thermotoga maritima |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 95 | activity range | Thermotoga maritima |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Thermotoga maritima |
General Information | Comment | Organism |
---|---|---|
evolution | Tm-RNase III cleaves an Ec-RNase III substrate with identical specificity and is inhibited by antideterminant bp that also inhibit Ec-RNase III, indicating the conservation, across a broad phylogenetic distance, of positive and negative determinants of reactivity of bacterial RNase III substrates | Thermotoga maritima |
physiological function | cleavage of double-stranded RNA by ribonuclease III is a conserved early step in bacterial rRNA maturation, mechanism of dsRNA cleavage by RNase III, overview | Thermotoga maritima |