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Literature summary for 3.1.26.3 extracted from

  • Dalzell, J.J.; Warnock, N.D.; Stevenson, M.A.; Mousley, A.; Fleming, C.C.; Maule, A.G.
    Short interfering RNA-mediated knockdown of drosha and pasha in undifferentiated Meloidogyne incognita eggs leads to irregular growth and embryonic lethality (2010), Int. J. Parasitol., 40, 1303-1310.
    View publication on PubMed
Search for more literature for 3.1.26.3

Activating Compound

Activating Compound Comment Organism Structure
additional information silencing of the drosha cofactor, pasha, leads to developmental complications and consequent lethality Meloidogyne incognita

Application

Application Comment Organism
additional information drosha, pasha and their ancillary factors may represent excellent targets for novel nematicides and/or in planta controls, and potentially other parasitic nematodes, through disruption of micro-RNA-directed developmental pathways Meloidogyne incognita

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol dicer Meloidogyne incognita 5829
-
nucleus drosha Meloidogyne incognita 5634
-

Organism

Organism UniProt Comment Textmining
Meloidogyne incognita
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
egg
-
 Meloidogyne incognita
-

Synonyms

Synonyms Comment Organism
Dicer
-
 Meloidogyne incognita
Drosha
-
 Meloidogyne incognita
RNase III
-
 Meloidogyne incognita

Expression

Organism Comment Expression
Meloidogyne incognita Micro-drosha siRNAs-1 to -3 yield a very highly significant level of target transcript knockdown compared with the control siRNA following egg-stage soaks, with a mean 88.1%, 98.6%, and 99.89% decrease, respectively. Soaking the J2s in dicer siRNA results in a modest decrease in dicer transcript abundance down
Meloidogyne incognita Micro-dicer siRNAs-1 to -3 elicit percentage transcript increases of 775, 1832 and 645, respectively up

General Information

General Information Comment Organism
malfunction on silencing drosha, a critical effector of micro-RNA maturation, significant inhibition of normal development and hatching in short interfering (si)RNA-soaked eggs. Drosha knockdown proves embryonically lethal. Impact of silencing dicer substantial up-regulates dicer transcript abundance, which does not impact on egg differentiation or hatching rates. Soaking the J2s in dicer siRNA results in a modest decrease in dicer transcript abundance which has no observable impact on phenotype or behaviour within 48 h of initial exposure to siRNA Meloidogyne incognita