Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) | Rock bream iridovirus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NaCl | completely inhibited at 200 mM NaCl (within physiological ranges) irrespective of Mg2+ concentrations (0.5-20 mM) | Rock bream iridovirus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | dsRNA substrate is cleaved by the RBIV RNase III at high concentrations of Mg2+ (5-20 mM) and at low salt concentration (50 mM) | Rock bream iridovirus | |
Mn2+ | substrate dsRNA is cleaved at low concentrations of Mn2+ (0.5-1 mM) at low salt concentration (50 mM) and is cleaved by increasing Mn2+ (5-20 mM) at 200 mM salt | Rock bream iridovirus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30900 | - |
calculated from sequence | Rock bream iridovirus |
31090 | - |
of the His-tag fused protein, SDS-PAGE | Rock bream iridovirus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rock bream iridovirus | Q5YF04 | RBIV | - |
Rock bream iridovirus RBIV | Q5YF04 | RBIV | - |
Purification (Comment) | Organism |
---|---|
six-His fusion protein is purified from the soluble fraction using Ni-nitrilotriacetic resin | Rock bream iridovirus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
double-stranded RNA + H2O | - |
Rock bream iridovirus | 5'-phosphooligonucleotides | - |
? | |
double-stranded RNA + H2O | - |
Rock bream iridovirus RBIV | 5'-phosphooligonucleotides | - |
? | |
dsDNA + H2O | - |
Rock bream iridovirus | ? | - |
? | |
dsDNA + H2O | - |
Rock bream iridovirus RBIV | ? | - |
? | |
single-stranded RNA + H2O | - |
Rock bream iridovirus | 5'-phosphooligonucleotides | - |
? | |
single-stranded RNA + H2O | - |
Rock bream iridovirus RBIV | 5'-phosphooligonucleotides | - |
? |
Synonyms | Comment | Organism |
---|---|---|
RBIV RNase III | - |
Rock bream iridovirus |
RNase III | - |
Rock bream iridovirus |