Crystallization (Comment) | Organism |
---|---|
12 three-dimensional structures of bacterial RNase III in various forms have been reported | Escherichia coli |
12 three-dimensional structures of bacterial RNase III in various forms have been reported | Aquifex aeolicus |
in complex with dsRNA, in the presence of Mg2+ or Mn2+ one metal ion bound per polypeptide chain | Aquifex aeolicus |
RNA-free structure of full length RNase III, comparison with crystal structures of Aquifex aeolicus RNase-dsRNA complex indicates dramatic conformational changes upon dsRNA binding | Thermotoga maritima |
Protein Variants | Comment | Organism |
---|---|---|
D44N | mutation of the cleavage site | Aquifex aeolicus |
E110A | uncoupling of the dsRNA-binding and processing abilities of the enzyme | Aquifex aeolicus |
E110K | loss of Mg2+ binding capacity, non-functional, uncoupling of the dsRNA-binding and processing abilities of the enzyme | Aquifex aeolicus |
E110K | mutation of the cleavage site | Aquifex aeolicus |
E110Q | uncoupling of the dsRNA-binding and processing abilities of the enzyme | Aquifex aeolicus |
Q153P | the Q153P substitution in the middle of the flexible linker between the endoND and the dsRBD abolish RNA-cleavage activity | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | inactive | Escherichia coli | |
Co2+ | can substitute for Mg2+ | Escherichia coli | |
Mg2+ | - |
Escherichia coli | |
Mg2+ | - |
Aquifex aeolicus | |
Mg2+ | - |
Homo sapiens | |
Mg2+ | - |
Saccharomyces cerevisiae | |
Mg2+ | required | Escherichia coli | |
Mg2+ | required | Aquifex aeolicus | |
Mn2+ | - |
Aquifex aeolicus | |
Mn2+ | can substitute for Mg2+ | Escherichia coli | |
Ni2+ | can substitute for Mg2+ | Escherichia coli | |
Sr2+ | inactive | Escherichia coli | |
Zn2+ | inactive | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
double-stranded RNA + H2O | Aquifex aeolicus | responsible for processing of dsRNA | 5'-phosphooligonucleotides | - |
? | |
double-stranded RNA + H2O | Thermotoga maritima | responsible for processing of dsRNA | 5'-phosphooligonucleotides | small duplex products of 10-18 base pairs | ? | |
double-stranded RNA + H2O | Escherichia coli | responsible for processing of dsRNA | 5'-phosphooligonucleotides | small duplex products of 10-18 base pairs | ? | |
additional information | Escherichia coli | as a binding protein, RNase III binds and stabilizes certain RNAs, thus suppressing the expression of certain genes | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aquifex aeolicus | O67082 | - |
- |
Escherichia coli | P0A7Y0 | - |
- |
Escherichia coli | P0A7Y0 | K12 | - |
Homo sapiens | Q9NRR4 | - |
- |
Homo sapiens | Q9UPY3 | - |
- |
Saccharomyces cerevisiae | Q02555 | - |
- |
Thermotoga maritima | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
double-stranded RNA + H2O | responsible for processing of dsRNA | Aquifex aeolicus | 5'-phosphooligonucleotides | - |
? | |
double-stranded RNA + H2O | responsible for processing of dsRNA | Thermotoga maritima | 5'-phosphooligonucleotides | small duplex products of 10-18 base pairs | ? | |
double-stranded RNA + H2O | responsible for processing of dsRNA | Escherichia coli | 5'-phosphooligonucleotides | small duplex products of 10-18 base pairs | ? | |
additional information | as a binding protein, RNase III binds and stabilizes certain RNAs, thus suppressing the expression of certain genes | Escherichia coli | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Thermotoga maritima |
dimer | - |
Escherichia coli |
dimer | - |
Aquifex aeolicus |
Synonyms | Comment | Organism |
---|---|---|
Aa-RNase III | - |
Aquifex aeolicus |
Dicer | - |
Homo sapiens |
Drosha | - |
Homo sapiens |
Ec-RNase III | - |
Escherichia coli |
Hs-Dicer | - |
Homo sapiens |
Hs-Drosha | - |
Homo sapiens |
ribonuclease III | - |
Aquifex aeolicus |
ribonuclease III | - |
Homo sapiens |
RNase III | - |
Escherichia coli |
RNase III | - |
Aquifex aeolicus |
RNase III | - |
Homo sapiens |
Rnt1p | - |
Saccharomyces cerevisiae |
Sc-Rnt1p | - |
Saccharomyces cerevisiae |
Tm-RNase III | - |
Thermotoga maritima |