Literature summary for 3.1.26.3 extracted from

Gan, J.; Tropea, J.E.; Austin, B.P.; Court, D.L.; Waugh, D.S.; Ji, X.
Structural insight into the mechanism of double-stranded RNA processing by ribonuclease III (2006), Cell, 124, 355-366.

Search for more literature for 3.1.26.3

Cloned(Commentary)

Cloned (Comment)	Organism
expression of mutant enzyme D44N in Escherichia coli	Aquifex aeolicus
the full-length Aa-D44N is cloned for overproduction of the enzyme in Escherichia coli BL21DE3 CodonPlus-RIL cells	Aquifex aeolicus

Crystallization (Commentary)

Crystallization (Comment)	Organism
crystal structure of Aquifex aeolicus mutant enzyme D44N with double-stranded RNA bound in its catalytic valley	Aquifex aeolicus
the structure of Aa-D44N RNase III in complex with a RNA product is determined at a resolution of 2.05 A	Aquifex aeolicus

Protein Variants

Protein Variants	Comment	Organism
D44N	mutant enzyme with greatly reduced activity	Aquifex aeolicus
D44N	mutant with greatly reduced activity	Aquifex aeolicus
E110Q	mutant enzyme has negligible RNA cleavage activity but retains its RNA binding affinity	Aquifex aeolicus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	-	Aquifex aeolicus

Organism

Organism	UniProt	Comment	Textmining
Aquifex aeolicus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Aquifex aeolicus
mutant enzyme D44N	Aquifex aeolicus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
double-stranded RNA + H2O	RNA 5, a 30 base stem-loop RNA of the sequence 5 '-AUAAAGGUCAUUCGCAAGAGUGGCCUUUAU-3', is cleaved by RNase III (D44N) from Aquifex aeolicus. The products of the reaction include a dinucleotide 5'-AU-3' and a 28 base stem-loop RNA with a two-base 3' overhang (RNA 6). Two RNA 6 molecules and a dimeric mutant enzyme D44N molecule form a product complex	Aquifex aeolicus	?	-	?

Synonyms

Synonyms	Comment	Organism
Aa-RNase III	-	Aquifex aeolicus
ribonuclease III	-	Aquifex aeolicus
RNase III	-	Aquifex aeolicus

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Release 2023.1 (January 2023)