Cloned (Comment) | Organism |
---|---|
gene rnc, mapping at 55 min on the bacterial chromosome, overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.26 | - |
RNA | 37°C | Escherichia coli | |
0.34 | - |
RNA | recombinant His-tagged enzyme, pH 8.0, 35°C | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | can partially substitute for Mg2+ | Escherichia coli | |
Mg2+ | dependent on | Escherichia coli | |
Mn2+ | can partially substitute for Mg2+ | Escherichia coli | |
additional information | no activity with Ca2+ and Sr2+ | Escherichia coli | |
Ni2+ | can partially substitute for Mg2+ | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25600 | - |
x * 25600, amino acid sequence calculation, x * 29000, recombinant His-tagged enzyme, SDS-PAGE | Escherichia coli |
29000 | - |
x * 25600, amino acid sequence calculation, x * 29000, recombinant His-tagged enzyme, SDS-PAGE | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dsRNA + H2O | Escherichia coli | - |
processed RNA | - |
? | |
additional information | Escherichia coli | involved in the maturation of the ribosomal RNA precursor, and bacteriophage T7 mRNA precursors, enzyme participates in the degradation as well as maturation of diverse cellular, phage, and plasmid RNAs | ? | - |
? | |
pre-16S rRNA + H2O | Escherichia coli | - |
mature 16S rRNA | - |
? | |
pre-23S rRNA + H2O | Escherichia coli | - |
mature 23S rRNA | - |
? | |
pre-5S rRNA + H2O | Escherichia coli | - |
mature 5S rRNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme to over 90% purity | Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
Endonucleolytic cleavage to a 5'-phosphomonoester | determination of specific cleavage site, Glu117 is important for phosphodiester hydrolysis | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
gel mobility shift assay for substrate bindng determination | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dsRNA + H2O | - |
Escherichia coli | processed RNA | - |
? | |
dsRNA + H2O | specific for double-stranded RNA | Escherichia coli | processed RNA | enzyme produces 12-15 base pair duplex products with 5'-phosphate, 3'-hydroxyl termini | ? | |
additional information | involved in the maturation of the ribosomal RNA precursor, and bacteriophage T7 mRNA precursors, enzyme participates in the degradation as well as maturation of diverse cellular, phage, and plasmid RNAs | Escherichia coli | ? | - |
? | |
pre-16S rRNA + H2O | - |
Escherichia coli | mature 16S rRNA | - |
? | |
pre-23S rRNA + H2O | - |
Escherichia coli | mature 23S rRNA | - |
? | |
pre-5S rRNA + H2O | - |
Escherichia coli | mature 5S rRNA | - |
? | |
R1.1 RNA + H2O | substrate is enzymatically synthesized based on the R1.1 processing signal, which is encoded in the phage T7 genetic early region between genes 1.0 and 1.1, 1 cleavage site | Escherichia coli | 2 fragment of R1.1 RNA | - |
? | |
RNA + H2O | - |
Escherichia coli | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 25600, amino acid sequence calculation, x * 29000, recombinant His-tagged enzyme, SDS-PAGE | Escherichia coli |
More | the enzyme contains a dsRNA binding and a catalytic subdomain | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
More | enzyme belongs to the ribonuclease III superfamily | Escherichia coli |
RNase III | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
assay at | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.8 | - |
RNA | recombinant His-tagged enzyme, pH 8.0, 35°C | Escherichia coli | |
7.7 | - |
RNA | 37°C | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Escherichia coli |