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Literature summary for 3.1.26.3 extracted from

  • Srivastava, N.; Srivastava, R.A.
    Expression, purification and properties of recombinant E. coli ribonuclease III (1996), Biochem. Mol. Biol. Int., 39, 171-180.
    View publication on PubMed
Search for more literature for 3.1.26.3

Activating Compound

Activating Compound Comment Organism Structure
NH4+ stimualtion at 20 mM Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of the rnc gene in Escherichia coli Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
Mn2+ at concentrations higher than 0.5 mM Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
K+ stimulation at 20 mM Escherichia coli
Mg2+ required Escherichia coli
Mn2+ stimulates Escherichia coli
Mn2+ optimal concentration at 0.15-0.3 mM Escherichia coli
Na+ stimualtion at 20 mM Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
double-stranded RNA + H2O Escherichia coli cleaves multimeric tRNA precursor at the spacer region, also involved in processing of precursor rRNA, hnRNA and early T7-mRNA. Also cleaves double-stranded DNA and single-stranded RNA 5'-phosphooligonucleotides
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
of the overexpressed protein Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
double-stranded RNA + H2O
-
 Escherichia coli 5'-phosphooligonucleotides
-
 ?
double-stranded RNA + H2O cleaves multimeric tRNA precursor at the spacer region, also involved in processing of precursor rRNA, hnRNA and early T7-mRNA. Also cleaves double-stranded DNA and single-stranded RNA Escherichia coli 5'-phosphooligonucleotides
-
 ?
poly(A)-poly(U) + H2O
-
 Escherichia coli 5'-phosphooligonucleotides
-
 ?
ribosomal RNA + H2O the small and stable 10Sa RNA is cleaved Escherichia coli smaller precursor rRNA
-
 ?
ribosomal RNA + H2O 7S RNA is cleaved Escherichia coli smaller precursor rRNA
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 30 min, no loss af activity Escherichia coli
50
-
 30 min, 50% loss af activity Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
6.9 7.4
-
 Escherichia coli