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Literature summary for 3.1.26.11 extracted from
- Residues in two homology blocks on the amino side of the tRNase Z His domain contribute unexpectedly to pre-tRNA 3' end processing (2006), RNA, 12, 1104-1115.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Drosophila melanogaster |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C442A | 12fold reduction of processing efficiency compared to wild-type | Drosophila melanogaster |
| C467A | 8fold reduction of processing efficiency compared to wild-type | Drosophila melanogaster |
| D466A | 1500fold reduction of processing efficiency compared to wild-type | Drosophila melanogaster |
| E469A | 300fold reduction of processing efficiency compared to wild-type | Drosophila melanogaster |
| G438A | 5000fold reduction of processing efficiency compared to wild-type | Drosophila melanogaster |
| G440A | 4fold reduction of processing efficiency compared to wild-type | Drosophila melanogaster |
| G468A | 900fold reduction of processing efficiency compared to wild-type | Drosophila melanogaster |
| G470A | 13fold reduction of processing efficiency compared to wild-type | Drosophila melanogaster |
| G473A | 8fold reduction of processing efficiency compared to wild-type | Drosophila melanogaster |
| G480A | 8fold reduction of processing efficiency compared to wild-type | Drosophila melanogaster |
| I443A | 8fold reduction of processing efficiency compared to wild-type | Drosophila melanogaster |
| I475A | 5fold reduction of processing efficiency compared to wild-type | Drosophila melanogaster |
| K446A | 25fold reduction of processing efficiency compared to wild-type | Drosophila melanogaster |
| L437A | 7fold reduction of processing efficiency compared to wild-type | Drosophila melanogaster |
| L464A | 5fold reduction of processing efficiency compared to wild-type | Drosophila melanogaster |
| L465A | 10fold reduction of processing efficiency compared to wild-type | Drosophila melanogaster |
| L478A | 11fold reduction of processing efficiency compared to wild-type | Drosophila melanogaster |
| N445A | 5fold reduction of processing efficiency compared to wild-type | Drosophila melanogaster |
| N449A | 80fold reduction of processing efficiency compared to wild-type | Drosophila melanogaster |
| P444A | 5fold reduction of processing efficiency compared to wild-type | Drosophila melanogaster |
| Q474A | 750fold reduction of processing efficiency compared to wild-type | Drosophila melanogaster |
| R448A | 625fold reduction of processing efficiency compared to wild-type | Drosophila melanogaster |
| R477A | 2600fold reduction of processing efficiency compared to wild-type | Drosophila melanogaster |
| S441A | 9fold reduction of processing efficiency compared to wild-type | Drosophila melanogaster |
| T439A | 2.5fold reduction of processing efficiency compared to wild-type | Drosophila melanogaster |
| T447A | 2fold reduction of processing efficiency compared to wild-type | Drosophila melanogaster |
| T471A | 5fold reduction of processing efficiency compared to wild-type | Drosophila melanogaster |
| V450A | 4fold reduction of processing efficiency compared to wild-type | Drosophila melanogaster |
| V463A | 6fold reduction of processing efficiency compared to wild-type | Drosophila melanogaster |
| V476A | 6fold reduction of processing efficiency compared to wild-type | Drosophila melanogaster |
| Y472A | 4fold reduction of processing efficiency compared to wild-type | Drosophila melanogaster |
| Y479A | 7fold reduction of processing efficiency compared to wild-type | Drosophila melanogaster |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0000198 | - |
pre-tRNAHis | - |
Drosophila melanogaster | |
| 0.0000394 | - |
pre-tRNAHis-AUG | - |
Drosophila melanogaster | |
| 0.0000401 | - |
pre-tRNAHis-CCA | - |
Drosophila melanogaster |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Drosophila melanogaster | Q8MKW7 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Drosophila melanogaster |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | - |
Drosophila melanogaster | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pre-tRNAHis + H2O | - |
Drosophila melanogaster | ? | - |
? |  |
| pre-tRNAHis-AUG + H2O | - |
Drosophila melanogaster | ? | - |
? | |
| pre-tRNAHis-CCA + H2O | - |
Drosophila melanogaster | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| tRNase Z | - |
Drosophila melanogaster |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0049 | - |
pre-tRNAHis-CCA | - |
Drosophila melanogaster | |
| 0.083 | - |
pre-tRNAHis | - |
Drosophila melanogaster | |
| 0.365 | - |
pre-tRNAHis-AUG | - |
Drosophila melanogaster |
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