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Literature summary for 3.1.26.11 extracted from
- Endonucleolytic processing of CCA-less tRNA precursors by RNase Z in Bacillus subtilis (2003), EMBO J., 22, 4534-4543.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Bacillus subtilis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00058 | - |
trnI-Thr-TCAATG-trailer | - |
Bacillus subtilis | |
| 0.00097 | - |
trnI-Thr-TAAATG-trailer | native trailer sequence | Bacillus subtilis | |
| 0.0022 | - |
trnI-Thr-CAAATG-trailer | the first cytosine is the primary anti-determinant for RNaseZ | Bacillus subtilis | |
| 0.0024 | - |
trnI-Thr-CCAATG-trailer | CCA motif inhibits RNaseZ cleavage | Bacillus subtilis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| His-tag fusion, Ni-affinity chromatography | Bacillus subtilis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | - |
Bacillus subtilis | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pTyrI + H2O | pre-tRNATyr from Oenothera berteriana | Bacillus subtilis | ? | - |
? |  |
| trnB-THr + H2O | pre-tRNATHr with a 47 nucleotide 3' trailing sequence and CCA motif, no cleavage | Bacillus subtilis | ? | - |
? | |
| trnI-Thr + H2O | pre-tRNAThr with a 83 nucleotide 3' trailing sequence, cleavage at one or two bases downstream the discriminator base | Bacillus subtilis | ? | - |
? | |
| trnI-Thr-CAAATG-trailer + H2O | - |
Bacillus subtilis | ? | - |
? | |
| trnI-Thr-CCAATG-trailer + H2O | - |
Bacillus subtilis | ? | - |
? | |
| trnI-Thr-TAAATG-trailer + H2O | native trailer sequence | Bacillus subtilis | ? | - |
? | |
| trnI-Thr-TCAATG-trailer + H2O | - |
Bacillus subtilis | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 3' tRNase | - |
Bacillus subtilis |
| RNaseZ | - |
Bacillus subtilis |
| YqjK | - |
Bacillus subtilis |
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Release 2023.1 (January 2023)
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