Literature summary for 3.1.21.3 extracted from

Uyen, N.T.; Park, S.Y.; Choi, J.W.; Lee, H.J.; Nishi, K.; Kim, J.S.
The fragment structure of a putative HsdR subunit of a type I restriction enzyme from Vibrio vulnificus YJ016: implications for DNA restriction and translocation activity (2009), Nucleic Acids Res., 37, 6960-6969.

Cloned(Commentary)

Cloned (Comment)	Organism
full length HsdR transformed into Escherichia coli B834(DE3) cells	Vibrio vulnificus YJ016

Crystallization (Commentary)

Crystallization (Comment)	Organism
N-terminal fragment (ca. 590 amino acids) of a putative HsdR subunit (817 amino acids), native crystals and Se-Met crystals, to 2.5 A resolution, by hanging-drop vapor-diffusion method at 22°C. Se-Met crystal belongs to the orthorhombic space group P212121 with unit-cell dimensions of a = 71.01, b = 89.04 and c = 113.66 A. Seven Se sites in the asymmetric unit at 3.0 A resolution. Crystal structure reveals catalytic sites for nuclease (NTD-domain, Ala21-Ile159) and ATPase (RecA-like domains: RD1 for Lys160-Tyr360 and RD2 for Ala372-Val590) activities and suggests a possible translocation mechanism of the HsdR subunit	Vibrio vulnificus YJ016

Crystallization (Comment)

Organism

N-terminal fragment (ca. 590 amino acids) of a putative HsdR subunit (817 amino acids), native crystals and Se-Met crystals, to 2.5 A resolution, by hanging-drop vapor-diffusion method at 22°C. Se-Met crystal belongs to the orthorhombic space group P212121 with unit-cell dimensions of a = 71.01, b = 89.04 and c = 113.66 A. Seven Se sites in the asymmetric unit at 3.0 A resolution. Crystal structure reveals catalytic sites for nuclease (NTD-domain, Ala21-Ile159) and ATPase (RecA-like domains: RD1 for Lys160-Tyr360 and RD2 for Ala372-Val590) activities and suggests a possible translocation mechanism of the HsdR subunit

Vibrio vulnificus YJ016

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
60000	-	x * 90000, full length protein, SDS-PAGE. X * 60000, crystallized HsdR subunit, SDS-PAGE. X * 95000, putative HsdR subunit, calculated from sequence	Vibrio vulnificus YJ016
90000	-	x * 90000, full length protein, SDS-PAGE. X * 60000, crystallized HsdR subunit, SDS-PAGE. X * 95000, putative HsdR subunit, calculated from sequence	Vibrio vulnificus YJ016
95000	-	x * 90000, full length protein, SDS-PAGE. X * 60000, crystallized HsdR subunit, SDS-PAGE. X * 95000, putative HsdR subunit, calculated from sequence	Vibrio vulnificus YJ016

Organism

Organism	UniProt	Comment	Textmining
Vibrio vulnificus YJ016	Q7MPU7	-	-

Purification (Commentary)

Purification (Comment)	Organism
native HsdR protein homogeneously purified by sequential chromatographic steps	Vibrio vulnificus YJ016

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
DNA + H2O	-	Vibrio vulnificus YJ016	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 90000, full length protein, SDS-PAGE. X * 60000, crystallized HsdR subunit, SDS-PAGE. X * 95000, putative HsdR subunit, calculated from sequence	Vibrio vulnificus YJ016

Synonyms

Synonyms	Comment	Organism
type I restriction enzyme	-	Vibrio vulnificus YJ016