Cloned (Comment) | Organism |
---|---|
EcoR124I HsdR with selenomethionine labeling expressed in Escherichia coli | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
motor subunit HsdR of pR124 plasmid-borne type I restriction-modification enzyme EcoR124I, solved in complex with Mg2+-ATP at 2.6 A resolution. HsdR presents four globular domains in a square-planar arrangement, generating prominent grooves between adjacent domain pairs. Lys220 on alpha8 is 3.1 A from N3 on the exposed edge of ATP bound at the helicase domains, potentially coupling endonuclease and helicase function. A uniformly positive surface groove with a clear match to the size and shape of duplex DNA proceeds from a canonical helicase cleft in a continuous path down the front of the motor subunit between the helical and endonuclease domains, where the cleavage site is recessed slightly from the surface | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | Q304R3 | - |
- |
Subunits | Comment | Organism |
---|---|---|
pentamer | crystallography | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
EcoR124I | - |
Escherichia coli |
type I restriction-modification enzyme | - |
Escherichia coli |