Application | Comment | Organism |
---|---|---|
additional information | transcription of both the restriction endonuclease and methyltransferase genes is controlled by interdependent regulatory loops governed by the C.Esp1396I protein | synthetic construct |
Cloned (Comment) | Organism |
---|---|
esp1396I genes expressed in Escherichia coli HB101 and XL1-Blue | synthetic construct |
Protein Variants | Comment | Organism |
---|---|---|
additional information | mutations within the tetranucleotide repeats diminish binding of C.Esp1396I. Insertion of an additional base into the TATA sequence to make the GACT/AGCT sequences symmetric around a TAT spacer reduces DNA binding | synthetic construct |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | a repressing complex of C.Esp1396I tetramer bound to both binding sites | synthetic construct |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
17920 | - |
sedimentation equilibrium analytical ultracentrifugation of C.Esp1396I | synthetic construct |
18450 | - |
theoretical Mr of C.Esp1396I | synthetic construct |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | synthetic construct | basal transcription from promoter esp1396ICR results in gradual accumulation of C.Esp1396I, which upon dimerization binds to a single site in promoter esp1396IM, preventing further transcription from this promoter. Further accumulation of C.Esp1396I results in activation and then gradual repression of promoter esp1396ICR | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
synthetic construct | - |
- |
- |
Purification (Comment) | Organism |
---|---|
C.Esp1396I-6His and C.Esp1396I without hexahistidine tag | synthetic construct |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | - |
synthetic construct | ? | - |
? | |
additional information | basal transcription from promoter esp1396ICR results in gradual accumulation of C.Esp1396I, which upon dimerization binds to a single site in promoter esp1396IM, preventing further transcription from this promoter. Further accumulation of C.Esp1396I results in activation and then gradual repression of promoter esp1396ICR | synthetic construct | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | sedimentation equilibrium analytical ultracentrifugation of C.Esp1396I | synthetic construct |
Synonyms | Comment | Organism |
---|---|---|
Esp1396I | - |
synthetic construct |