Cloned (Comment) | Organism |
---|---|
HsdR subunit cloned into pProExHTc and expressed in Escherichia coli B834(DE3) | Vibrio vulnificus YJ016 |
Crystallization (Comment) | Organism |
---|---|
hanging-drop and sitting-drop vapour-diffusion method, HsdR subunit crystallized from 8%(w/v) polyethylene glycol 3350, 0.15 M ammonium chloride, 0.1 M HEPES pH 7.5 and 2 mM beta-mercaptoethanol, to 2.60 A resolution. Crystal belongs to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 71.01, b = 89.04, c = 113.66 A. With one HsdR molecule in the asymmetric unit, the Matthews coefficient is 2.14 A3 Da-1 and the solvent content is 42% | Vibrio vulnificus YJ016 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vibrio vulnificus YJ016 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
HsdR fusion protein purified by gel filtration. HsdR with five additional amino acids (YFQGA) at the N-terminus purified on anion-exchange column and by gel filtration, purified protein is more than 95% pure | Vibrio vulnificus YJ016 |
Synonyms | Comment | Organism |
---|---|---|
type I restriction enzyme | - |
Vibrio vulnificus YJ016 |