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Literature summary for 3.1.21.3 extracted from
- The type I restriction endonuclease R.EcoR124I: Over-production and biochemical properties (1996), J. Mol. Biol., 257, 977-991.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| S-adenosyl-L-methionine | stimulates, not required | Escherichia coli |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| DNA | cleavage of DNA is inhibited by an increased degree of negative supercoiling | Escherichia coli | |
| S-adenosyl homocysteine | competitive | Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 290000 | 315000 | pentameric enzyme form R2M2S1, gel filtration | Escherichia coli |
| 312000 | - |
gel filtration | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
enzyme R.EcoR124I | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| duplex DNA + ATP | the enzyme is both a restriction endonuclease and a modification methylase. Hemi-methylated DNA is the preferred substrate for methylation | Escherichia coli | double-stranded DNA fragments with terminal 5'-phosphate + ADP + inorganic phosphate | - |
? | |
| duplex DNA + ATP | supercoiled with one or two SR124I recognition sites is cleaved by the same mechanism. Nicked-circle DNA is an intermediate of the cleavage reaction | Escherichia coli | double-stranded DNA fragments with terminal 5'-phosphate + ADP + inorganic phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | the stoichiometry of R.EcoR124 appears to be R1M2S1, the stoichiometry of R.EcoKI is R2M2S1. S is the HsdS-subunit which is responsible for DNA recognition, R is HsdR-subunit which is required for restriction and M is an independent methyltransferase, Mtase | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
EcoB, methylase activity | Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | required | Escherichia coli | |
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