Application | Comment | Organism |
---|---|---|
medicine | DNAse I/cdtB gene chimera has therapeutic applications for inhibiting the proliferation of cancer cells | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
DNAse I or DNAse I/cdtB gene chimera expressed from vectors pETBDN2 (cdB-DNAse I chimera), pETDNB1 (DNAse I-cdB chimera) and pETBDN2-1 to pETBDN2-5 (cdB-DNAse I mutated chimera) in Escherichia coli BL-21(DE3) | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
G55R | adds DNA contact residues, increases the specific activity of the hybrid protein | Homo sapiens |
L62E | added a metal ion-binding residue, increases the specific activity of the hybrid protein | Homo sapiens |
R100E | adds a residue that hydrogen bonds to the catalytic H160 in DNAse I, increases the specific activity of the hybrid protein | Homo sapiens |
S99Y | adds DNA contact residues, increases the specific activity of the hybrid protein | Homo sapiens |
V134R | adds DNA contact residues, increases the specific activity of the hybrid protein | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
actin | inhibits the DNA-nicking activity of DNAse I/CdtB chimera | Homo sapiens | |
additional information | G-actin has no effect on the ability of CdtB/DNAse I chimera to convert supercoiled DNA to relaxed and linear forms | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | P00639 | - |
- |
Homo sapiens | P24855 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
pancreas | - |
Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | - |
Homo sapiens | 5'-phosphooligonucleotides + ? | - |
? | |
DNA + H2O | DNAse I converts 100% of 1 microg of supercoiled plasmid DNA to relaxed form in 1 h at 37°C. A 10fold higher concentration of DNAse I converts 98% of 1 microg of supercoiled DNA to linear form and a 100fold higher concentration of the nuclease completely digests the DNA into small fragments | Bos taurus | 5'-phosphooligonucleotides + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterotrimer | CdtB/DNAse I, but not DNAseI/CdtB | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
DNase I | - |
Homo sapiens |
DNase I | - |
Bos taurus |
type I DNAse I | - |
Homo sapiens |
type I DNAse I | - |
Bos taurus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
100 | - |
DNAse I DNA nicking-activity is less than 50% of the control after 5 min of heating | Bos taurus |