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Literature summary for 3.1.21.1 extracted from

  • DiRienzo, J.M.; Cao, L.; Volgina, A.; Bandelac, G.; Korostoff, J..
    Functional and structural characterization of chimeras of a bacterial genotoxin and human type I DNAse (2009), FEMS Microbiol. Lett., 291, 222-231.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
medicine DNAse I/cdtB gene chimera has therapeutic applications for inhibiting the proliferation of cancer cells Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
DNAse I or DNAse I/cdtB gene chimera expressed from vectors pETBDN2 (cdB-DNAse I chimera), pETDNB1 (DNAse I-cdB chimera) and pETBDN2-1 to pETBDN2-5 (cdB-DNAse I mutated chimera) in Escherichia coli BL-21(DE3) Homo sapiens

Protein Variants

Protein Variants Comment Organism
G55R adds DNA contact residues, increases the specific activity of the hybrid protein Homo sapiens
L62E added a metal ion-binding residue, increases the specific activity of the hybrid protein Homo sapiens
R100E adds a residue that hydrogen bonds to the catalytic H160 in DNAse I, increases the specific activity of the hybrid protein Homo sapiens
S99Y adds DNA contact residues, increases the specific activity of the hybrid protein Homo sapiens
V134R adds DNA contact residues, increases the specific activity of the hybrid protein Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
actin inhibits the DNA-nicking activity of DNAse I/CdtB chimera Homo sapiens
additional information G-actin has no effect on the ability of CdtB/DNAse I chimera to convert supercoiled DNA to relaxed and linear forms Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus P00639
-
-
Homo sapiens P24855
-
-

Source Tissue

Source Tissue Comment Organism Textmining
pancreas
-
Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA + H2O
-
Homo sapiens 5'-phosphooligonucleotides + ?
-
?
DNA + H2O DNAse I converts 100% of 1 microg of supercoiled plasmid DNA to relaxed form in 1 h at 37°C. A 10fold higher concentration of DNAse I converts 98% of 1 microg of supercoiled DNA to linear form and a 100fold higher concentration of the nuclease completely digests the DNA into small fragments Bos taurus 5'-phosphooligonucleotides + ?
-
?

Subunits

Subunits Comment Organism
heterotrimer CdtB/DNAse I, but not DNAseI/CdtB Homo sapiens

Synonyms

Synonyms Comment Organism
DNase I
-
Homo sapiens
DNase I
-
Bos taurus
type I DNAse I
-
Homo sapiens
type I DNAse I
-
Bos taurus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
100
-
DNAse I DNA nicking-activity is less than 50% of the control after 5 min of heating Bos taurus