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Literature summary for 3.1.21.1 extracted from

  • Sato, S.; Fujita, K.; Kanazawa, M.; Mukumoto, K.; Ohtsuka, K.; Waki, M.; Takenaka, S.
    Electrochemical assay for deoxyribonuclease I activity (2008), Anal. Biochem., 381, 233-239.
    View publication on PubMed

Application

Application Comment Organism
analysis construction of a sensor electrode carrying Fc-oligo-SH to achieve an electrochemical DNase I assay. Under the optimum conditions of DNase I digestion at 37°C for 30 min, a quantitative analysis can be achieved in the range of 0.0001-0.01 units/microl of DNase I Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
EDTA current peaks of the Fc-oligo-SH-immobilized electrode are relatively stable within error before and after treatment of DNase I solution with EDTA or RNaseA solution, suggesting that this electrode can be used for the detection of DNase I activity specifically Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
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recombinant
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Fc-oligo-SH + H2O degradation of a thiolated ferrocenyloligonucleotide, efficiency of DNase I reaction on the electrode is 48, 72, or 73% when treated with 1 microl of 2, 1, or 0.5 micromol ferrocenyloligonucleotide, respectively. DNase I can cleave the oligonucleotide on the gold surface and does not show a nonspecific surface absorption Bos taurus ?
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Synonyms

Synonyms Comment Organism
deoxyribonuclease I
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Bos taurus
DNase I
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Bos taurus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Bos taurus