Application | Comment | Organism |
---|---|---|
analysis | construction of a sensor electrode carrying Fc-oligo-SH to achieve an electrochemical DNase I assay. Under the optimum conditions of DNase I digestion at 37°C for 30 min, a quantitative analysis can be achieved in the range of 0.0001-0.01 units/microl of DNase I | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | current peaks of the Fc-oligo-SH-immobilized electrode are relatively stable within error before and after treatment of DNase I solution with EDTA or RNaseA solution, suggesting that this electrode can be used for the detection of DNase I activity specifically | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
recombinant | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Fc-oligo-SH + H2O | degradation of a thiolated ferrocenyloligonucleotide, efficiency of DNase I reaction on the electrode is 48, 72, or 73% when treated with 1 microl of 2, 1, or 0.5 micromol ferrocenyloligonucleotide, respectively. DNase I can cleave the oligonucleotide on the gold surface and does not show a nonspecific surface absorption | Bos taurus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
deoxyribonuclease I | - |
Bos taurus |
DNase I | - |
Bos taurus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Bos taurus |