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Literature summary for 3.1.21.1 extracted from

  • O'Rourke, E.J.; Pinto, A.V.; Petroni, E.A.; Tolmasky, M.E.; Ielpi, L.
    Evidence for the active role of a novel nuclease from Helicobacter pylori in the horizontal transfer of genetic information (2004), J. Bacteriol., 186, 2586-2593.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.21.1

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol
-
 Helicobacter pylori
dithiothreitol
-
 Helicobacter pylori

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli as His-tag fusion construct Helicobacter pylori

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
17000
-
 SDS-PAGE Helicobacter pylori

Organism

Organism UniProt Comment Textmining
Helicobacter pylori
-
-
-

Purification (Commentary)

Purification (Comment) Organism
affinity chromatography Helicobacter pylori

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Helicobacter pylori
-

Storage Stability

Storage Stability Organism
-20, 50 mM Tris-HCl 10 mM 2-mercatpoethanol 100 mM NaCl 10 mM EDTA 50% glycerol, several months, minimal loss of activity Helicobacter pylori

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
double-stranded circular DNA + H2O
-
 Helicobacter pylori ?
-
 ?
double-stranded linear DNA + H2O
-
 Helicobacter pylori ?
-
 ?
single-stranded DNA + H2O
-
 Helicobacter pylori ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 17000, gel filtration Helicobacter pylori

Synonyms

Synonyms Comment Organism
NucT
-
 Helicobacter pylori

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Helicobacter pylori