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Literature summary for 3.1.21.1 extracted from
- Chicken deoxyribonuclease: purification, characterization, gene cloning and gene expression (2003), J. Protein Chem., 22, 41-49.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequences determination and analysis, expression in Escherichia coli BL21(DE3) in the insoluble fraction | Gallus gallus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | slight inhibition | Gallus gallus |  |
| iodoacetate | strong inhibition in presence of Cu2+ | Gallus gallus | |
| additional information | resistant to trypsin inactivation in absence of Ca2+ | Gallus gallus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | half-maximal activation at 4 mM | Gallus gallus | |
| Mn2+ | half-maximal activation at 1 mM | Gallus gallus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | - |
x * 30000, SDS-PAGE, x * 30859, isozyme A, mass spectrometry, x * 30882, isozyme B, mass spectrometry | Gallus gallus |
| 30859 | - |
x * 30000, SDS-PAGE, x * 30859, isozyme A, mass spectrometry, x * 30882, isozyme B, mass spectrometry | Gallus gallus |
| 30882 | - |
x * 30000, SDS-PAGE, x * 30859, isozyme A, mass spectrometry, x * 30882, isozyme B, mass spectrometry | Gallus gallus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Gallus gallus | Q9YGI5 | 2 isozymes A and B | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | binds to concanavalin A, contains an N-glycosylation site | Gallus gallus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 2479fold from pancreas, isozymes A and B to homogeneity | Gallus gallus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| endonucleolytic cleavage to 5'-phosphodinucleotide and 5'-phosphooligonucleotide end-products | preference for double-stranded DNA | Gallus gallus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| pancreas | - |
Gallus gallus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
purified isozymes A and B | Gallus gallus |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 30000, SDS-PAGE, x * 30859, isozyme A, mass spectrometry, x * 30882, isozyme B, mass spectrometry | Gallus gallus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DNase | - |
Gallus gallus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Gallus gallus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
inactivation above | Gallus gallus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8 | depending on the metal ion used | Gallus gallus |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Gallus gallus | isozyme B, isoelectric focusing | - |
4.43 |
| Gallus gallus | isozyme A, isoelectric focusing | - |
4.56 |
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