Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, expression in COS-7 cells | Canis lupus familiaris |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | complete inhibition at 20 mM | Canis lupus familiaris | |
EGTA | complete inhibition at 5 mM | Canis lupus familiaris |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates | Canis lupus familiaris | |
Mg2+ | activates | Canis lupus familiaris | |
Mn2+ | activates | Canis lupus familiaris | |
additional information | divalent cations are absolutely required for activity | Canis lupus familiaris |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Canis lupus familiaris | phylogenetic analysis | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Canis lupus familiaris | Q767J3 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | 1 potential N-glycosylation site | Canis lupus familiaris |
Purification (Comment) | Organism |
---|---|
260fold from pancreas, to homogeneity | Canis lupus familiaris |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
endonucleolytic cleavage to 5'-phosphodinucleotide and 5'-phosphooligonucleotide end-products | preference for double-stranded DNA | Canis lupus familiaris |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | low activity | Canis lupus familiaris | - |
large intestine | low activity | Canis lupus familiaris | - |
liver | low activity | Canis lupus familiaris | - |
additional information | no activity in spleen, parotid gland, lung, heart, cerebellum, and cerebrum | Canis lupus familiaris | - |
pancreas | highest enzyme activity and expression | Canis lupus familiaris | - |
small intestine | low activity | Canis lupus familiaris | - |
stomach | - |
Canis lupus familiaris | - |
sublingual gland | low activity | Canis lupus familiaris | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
purified enzyme | Canis lupus familiaris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | - |
Canis lupus familiaris | 5'-phosphodinucleotide + 5'-phosphooligonucleotide | - |
? | |
additional information | phylogenetic analysis | Canis lupus familiaris | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DNase I | - |
Canis lupus familiaris |
pancreatic DNase I | - |
Canis lupus familiaris |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Canis lupus familiaris |