Literature summary for 3.1.21.1 extracted from

Mori, S.; Yasuda, T.; Takeshita, H.; Nakajima, T.; Nakazato, E.; Mogi, K.; Kaneko, Y.; Kishi, K.
Molecular, biochemical and immunological analyses of porcine pancreatic DNase I (2001), Biochim. Biophys. Acta, 1547, 275-287.

Search for more literature for 3.1.21.1

Cloned(Commentary)

Cloned (Comment)	Organism
DNA sequence determination and analysis	Sus scrofa

Protein Variants

Protein Variants	Comment	Organism
N110S	site-directed mutagenesis, exchange mutant has the same sequence as the rat and bovine enzymes and becomes insensitive to low pH like the rat and bovine enzymes	Sus scrofa
T178S	site-directed mutagenesis, similar to the wild-type enzyme in pH-sensitivity	Sus scrofa

Inhibitors

Inhibitors	Comment	Organism	Structure
EDTA	slight inhibition	Sus scrofa
EGTA	-	Sus scrofa
additional information	no inhibition by G-actin, due to exchange of Y65 to H65 and A114 to S114 compared to the other G-actin-sensitive mammalian enzymes	Sus scrofa

Organism

Organism	UniProt	Comment	Textmining
Sus scrofa	P11936	-	-

Purification (Commentary)

Purification (Comment)	Organism
26500fold from pancreas, to homogeneity	Sus scrofa

Source Tissue

Source Tissue	Comment	Organism	Textmining
kidney	low activity	Sus scrofa	-
liver	low activity	Sus scrofa	-
additional information	no activity in spleen, heart, lung, stomach, large intestine, cerebrum, cerebellum, submaxillary gland, and thymus	Sus scrofa	-
pancreas	-	Sus scrofa	-
parotid gland	low activity	Sus scrofa	-
small intestine	low activity	Sus scrofa	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	purified enzyme	Sus scrofa

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
dsDNA + H2O	-	Sus scrofa	5'-phosphodinucleotide + 5'-phosphooligonucleotide	-	?

Synonyms

Synonyms	Comment	Organism
DNase I	-	Sus scrofa

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Sus scrofa

pH Range

pH Minimum	pH Maximum	Comment	Organism
additional information	-	sensitive to low pH, Asn110 is responsible	Sus scrofa

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Release 2023.1 (January 2023)