General Stability | Organism |
---|---|
prolonged storage in the frozen state in H2O causes a gradual decrease of activity, half life of DNases A and B is 2 months | Hordeum vulgare |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
E2-immunity protein | complete inhibition of activity on plasmid DNA | Hordeum vulgare |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | required for stability | Hordeum vulgare | |
Mg2+ | - |
Hordeum vulgare | |
Mn2+ | - |
Hordeum vulgare |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
SDS-PAGE, gel filtration | Hordeum vulgare |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
double-stranded DNA + H2O | Hordeum vulgare | involved in seed sprouting | 5'-phosphooligonucleotides + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hordeum vulgare | - |
2 active compounds, A and B | - |
Purification (Comment) | Organism |
---|---|
to homogeneity by chromatography steps | Hordeum vulgare |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seedling | - |
Hordeum vulgare | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | - |
Hordeum vulgare | 5'-phosphooligonucleotides + ? | - |
? | |
double-stranded DNA + H2O | involved in seed sprouting | Hordeum vulgare | 5'-phosphooligonucleotides + ? | - |
? | |
NO2-Ph-pdTp-NO2-Ph + H2O | - |
Hordeum vulgare | p-nitrophenol + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 32000, SDS-PAGE, gel filtration | Hordeum vulgare |
Synonyms | Comment | Organism |
---|---|---|
DNase A | - |
Hordeum vulgare |
DNase B | - |
Hordeum vulgare |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
in the presence of Mg2+, dependent on bivalent metal ions | Hordeum vulgare |
7.5 | - |
in the presence of Mn2+, different pH-optima, dependent on bivalent metal ions | Hordeum vulgare |