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Literature summary for 3.1.21.1 extracted from

  • Murai, K.; Yamanaka, M.; Akagi, K.; Anai, M.; Mukai, T.; Omae, T.
    Purification and properties of deoxyribonuclease from human urine (1978), Biochim. Biophys. Acta, 517, 186-194.
    View publication on PubMed
Search for more literature for 3.1.21.1

Inhibitors

Inhibitors Comment Organism Structure
G-actin 15% inactivation at 0.004 mg Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular secreted into urine Homo sapiens
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activation synergistic with Mg2+ Homo sapiens
Co2+
-
 Homo sapiens
Mg2+ activation synergistic with Ca2+ Homo sapiens
Mn2+
-
 Homo sapiens
Zn2+
-
 Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
 gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
double-stranded DNA + H2O Homo sapiens
-
 5'-phosphooligonucleotides + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
near homogeneity Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
urine
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA + H2O degrades native DNA 4-5times faster than denatured DNA Homo sapiens 5'-phosphooligonucleotides + ? mainly nucleotides larger than pentanucleotides ?
double-stranded DNA + H2O
-
 Homo sapiens 5'-phosphooligonucleotides + ?
-
 ?
relaxed circular plasmid DNA + H2O
-
 Homo sapiens 5'-phosphooligonucleotides + ?
-
 ?

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50
-
 stable up to Homo sapiens
60
-
 85% loss of activity after 5 min, complete inactivation after 10 min Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-20
-
 stable for 1 month Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.4
-
 in 33 mM sodium phosphate buffer Homo sapiens
5.5
-
 in 33 mM acetate buffer, 6 times faster than in phosphate buffer Homo sapiens
7
-
-
 Homo sapiens