General Stability | Organism |
---|---|
sensitives to repeated freezing and thawing | Coprinopsis cinerea |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EGTA | activity inhibited | Coprinopsis cinerea |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | in presence of Ca2+ less sensitive to buffer strength | Coprinopsis cinerea | |
Mg2+ | absolute requirement for activity, highest activity if both Mg2+ and Ca2+ are present | Coprinopsis cinerea |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
12000 | - |
SDS-PAGE | Coprinopsis cinerea |
14000 | - |
SDS-PAGE | Coprinopsis cinerea |
22000 | - |
gel filtration | Coprinopsis cinerea |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
double-stranded DNA + H2O | Coprinopsis cinerea | preference for single-stranded and supercoiled DNA | 5'-phosphooligonucleotides + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Coprinopsis cinerea | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
basidiocarp | - |
Coprinopsis cinerea | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
21 units/min/ng DNA digested | Coprinopsis cinerea |
Storage Stability | Organism |
---|---|
-70°C, 10 mM Tris/HCl, pH 7.4, 50% v/v glycerol, 2 years | Coprinopsis cinerea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
double-stranded DNA + H2O | preference for single-stranded and supercoiled DNA | Coprinopsis cinerea | 5'-phosphooligonucleotides + ? | - |
? | |
relaxed circular plasmid DNA + H2O | - |
Coprinopsis cinerea | 5'-phosphooligonucleotides + ? | - |
? | |
single-stranded circular DNA + H2O | preference for single-stranded regions | Coprinopsis cinerea | 5'-phosphooligonucleotides + ? | - |
? | |
supercoiled plasmid DNA + H2O | - |
Coprinopsis cinerea | linear DNA + ? | formation of a relaxed circle that subsequently converts to linear DNA if it is nicked on the other strand at the same position | ? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 12000 and 1 * 14000, gel filtration, SDS-PAGE | Coprinopsis cinerea |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Coprinopsis cinerea |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | 60 | activity remains unchanged | Coprinopsis cinerea |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
stable for 20 min | Coprinopsis cinerea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Coprinopsis cinerea |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | 64% activity below pH 5.0, 80% activity above pH 8.0 | Coprinopsis cinerea |