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Literature summary for 3.1.21.1 extracted from

  • Bellard, M.; Dretzen, G.; Giangrande, A.; Ramain, P.
    Nuclease digestion of transcriptionally active chromatin (1989), Methods Enzymol., 170, 317-346.
    View publication on PubMed
Search for more literature for 3.1.21.1

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ addition of Ca2+ stabilizes the enzyme, facilitates second cut in double-stranded DNA Bos taurus
Mg2+ presence of Mg2+ alone leads to cutting only one of the two strands Bos taurus
Mn2+
-
 Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
-
 Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
double-stranded DNA + H2O Bos taurus cutting one of the two strands by a nucleophilic attack on the O-3'-P-bond 5'-phosphooligonucleotides + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
pancreas
-
 Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
circular plasmid DNA + H2O
-
 Bos taurus 5'-phosphooligonucleotides + ?
-
 ?
DNA + H2O
-
 Bos taurus 5'-phosphooligonucleotides + ?
-
 ?
double-stranded DNA + H2O cutting one of the two strands by a nucleophilic attack on the O-3'-P-bond Bos taurus 5'-phosphooligonucleotides + ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
-
 Bos taurus